Intracellular redox potential is correlated with miRNA expression in MCF7 cells under hypoxic conditions

[en] Hypoxia is a ubiquitous feature of cancers, encouraging glycolytic metabolism, proliferation, and resistance to therapy. Nonetheless, hypoxia is a poorly defined term with confounding features described in the literature. Redox biology provides an important link between the external cellular microenvironment and the cell's response to changing oxygen pressures. In this paper, we demonstrate a correlation between intracellular redox potential (measured using optical nanosensors) and the concentrations of microRNAs (miRNAs) involved in the cell's response to changes in oxygen pressure. The correlations were established using surprisal analysis (an approach derived from thermodynamics and information theory). We found that measured redox potential changes reflect changes in the free energy computed by surprisal analysis of miRNAs. Furthermore, surprisal analysis identified groups of miRNAs, functionally related to changes in proliferation and metastatic potential that played the most significant role in the cell's response to changing oxygen pressure. © 2019 National Academy of Sciences. All rights reserved.

Research Center/Unit :

MolSys - Molecular Systems - ULiège

Disciplines :

Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others

Author, co-author :

Johnston, Hannah E.; School of Chemistry, University of Edinburgh, Edinburgh, EH9 3FJ, United Kingdom

Dickinson, Paul; Institute of Infection and Immunology Research, University of Edinburgh, Edinburgh, EH9 3FL, United Kingdom

Ivens, Alasdair C.; Institute of Infection and Immunology Research, University of Edinburgh, Edinburgh, EH9 3FL, United Kingdom

Buck, Amy H.; Institute of Infection and Immunology Research, University of Edinburgh, Edinburgh, EH9 3FL, United Kingdom

Levine, Raphaël David; Institute of Chemistry, Hebrew University of Jerusalem, Jerusalem, 91904, Israel

Remacle, Françoise ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de chimie physique théorique

Campbell, Colin J.; School of Chemistry, University of Edinburgh, Edinburgh, EH9 3FJ, United Kingdom

Language :

English

Title :

Intracellular redox potential is correlated with miRNA expression in MCF7 cells under hypoxic conditions

Publication date :

2019

Journal title :

Proceedings of the National Academy of Sciences of the United States of America

ISSN :

0027-8424

eISSN :

1091-6490

Publisher :

National Academy of Sciences

Volume :

116

Issue :

Pages :

19753-19759

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

Medical Research Scotland
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Leverhulme Trust

Available on ORBi :

since 05 January 2020

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