Reference : Effects of preservation methodology on stable isotope compositions of sea stars
Scientific journals : Article
Life sciences : Zoology
Life sciences : Environmental sciences & ecology
Life sciences : Aquatic sciences & oceanology
http://hdl.handle.net/2268/242541
Effects of preservation methodology on stable isotope compositions of sea stars
English
Le Bourg, Baptiste mailto [Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanographie biologique >]
Lepoint, Gilles mailto [Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Océanographie biologique >]
Michel, Loïc mailto [Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Département de Biologie, Ecologie et Evolution >]
Jan-2020
Rapid Communications in Mass Spectrometry
John Wiley & Sons
34
2
e8589
Yes (verified by ORBi)
International
0951-4198
1097-0231
Hoboken
NJ
[en] stable isotopes ; sea stars ; preservation ; isotopic niche
[en] Rationale:Stable isotope analysis is used to investigate the trophic ecology of organisms and, in order to use samples from archived collections, and it is important to know whether preservation methods alter the results. This study investigates the long-term effects of four preservation methods on sea stars isotopic composition and isotopic niche parameters.Methods:We assessed effects of preservation method (freezing, drying, formaldehyde, ethanol) and duration (0, 1, 3, 6, 9, 12 and 24 months) on the stable isotope ratios of carbon, nitrogen and sulfur of sea star tissues. Isotopic ratios were measured using CF-EA-IRMS. We also monitored the evolution ofcommonly used ecological metrics (isotopic niche parameters) throughout the experiment. Results:Clear changes of δ13C values were observed for samples stored in formaldehyde and ethanol. None of the preservation methods had significant or consistent effects on δ15Nvalues. Formaldehydeinduced a decrease of δ34S values. All these changes could be mitigated using correction factors.Isotopic niches parameters slightly changed over time when computed with δ13C and δ15N values, but inconsistent variations occurred when computed with δ13C and δ34S values.Conclusions: Overall, these results show that preservation may affect the stable isotope ratios of sea stars. Correction factors can be used to mitigate the effects of the preservation method on stable isotope ratios. Isotopic niches parameters are overall unchanged. Consequently, in most cases, museum samples are suitable to calculate isotopic niche parameters.
http://hdl.handle.net/2268/242541
10.1002/rcm.8589

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
Le Bourg_et_al-2020-Rapid_Communications_in_Mass_Spectrometry.pdfPublisher postprint1.36 MBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.