Identification and characterization of three ICRd repetitive elements in Gossypium D genome

[en] The activity of genome-specific repetitive sequences is the main cause of genome variation between Gossypium A and D genomes. Through comparative analysis of the two genomes, we retrieved a repetitive element termed ICRd motif, which appears frequently in the diploid Gossypium raimondii (D5) genome but rarely in the diploid Gossypium arboreum (A2) genome. We further explored the existence of the ICRd motif in chromosomes of G. raimondii, G. arboreum, and two tetraploid (AADD) cotton species, Gossypium hirsutum and Gossypium barbadense, by fluorescence in situ hybridization (FISH), and observed that the ICRd motif exists in the D5 and D-subgenomes but not in the A2 and A-subgenomes. The ICRd motif comprises two components, a variable tandem repeat (TR) region and a conservative sequence (CS). The two constituents each have hundreds of repeats that evenly distribute across 13 chromosomes of the D5 genome. The ICRd motif (and its repeats) was revealed as the common conservative region harbored by ancient Long Terminal Repeat Retrotransposons. Identification and investigation of the ICRd motif promotes the study of A and D genome differences, facilitates research on Gossypium genome evolution, and provides assistance to subgenome identification and genome assembling.

Disciplines :

Agriculture & agronomie

Auteur, co-auteur :

Lu, Hejun ; Université de Liège - ULiège > Terra

Cui, Xinglei

Zhao, Yanyan

Odongo Magwanga, Richard

Li, Pengcheng

Cai, Xiaoyan

Zhou, Zhongli

Wang, Xingxing

Liu, Yuling

Xu, Yanchao

Plus d'auteurs (12 en +)

Langue du document :

Anglais

Titre :

Identification and characterization of three ICRd repetitive elements in Gossypium D genome

Date de publication/diffusion :

2020

Titre du périodique :

PeerJ

eISSN :

2167-8359

Maison d'édition :

PeerJ, Etats-Unis - Californie

Peer reviewed :

Peer reviewed vérifié par ORBi

Disponible sur ORBi :

depuis le 12 décembre 2019

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Nombre de vues

148 (dont 13 ULiège)

Nombre de téléchargements

29 (dont 12 ULiège)

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