High-resolution melting of multiple barcode amplicons for plant species authentication.

[en] In recent years, species identification in herbs has attracted considerable attention due to several cases of fraud; hence inexpensive high-throughput authentication methods are highly welcomed. Species authentication is often performed through DNA analysis and several specific regions (barcodes) are considered suitable. Each barcode (Bar) possesses different qualities in terms of universality and discrimination power. A multiplexed format where information can be extracted simultaneously from several barcode regions is seemingly appropriate to ensure the power of both universality and discrimination. In this approach, we amplified DNA from five different barcode regions in a multiplexed PCR format followed by high-resolution melting (HRM). This multiplexed Bar-HRM approach was first applied to plants spanning the plant kingdom and then gradually narrowing down the genetic variability within the Lamiaceae and the Solanaceae families to finally reach closely related cultivars. Universality was demonstrated through distinct melting profiles obtained for species originating from 29 different families spanning the angiosperms, gymnosperm, mosses, and liverwort (Marchantiophyta). Discrimination power was retained for species, sub-species, and a few cultivars through the application of multivariate statistics to the high-resolution melting profiles. This preliminary investigation has shown the potential to discriminate a vast amount of species within the whole plant kingdom. It requires no a priori knowledge of the species' DNA sequence and occurs in a closed system within 2.5h at a reduced cost per sample compared to other DNA based approaches.

Disciplines :

Genetics & genetic processes

Author, co-author :

Ballin, Nicolai Zederkopff

Onaindia, Jone Omar

Jawad, Hadeel

FERNANDEZ CARAZO, Rafael ; Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire génétique moléculaire

Maquet, Alain

Language :

English

Title :

High-resolution melting of multiple barcode amplicons for plant species authentication.

Publication date :

2019

Journal title :

Food Control

ISSN :

0956-7135

eISSN :

1873-7129

Publisher :

Elsevier, Netherlands

Volume :

105

Pages :

141-150

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 14 November 2019

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