Formation of the 67-kDa laminin receptor by acylation of the precursor.

Acylation; Antigens, Differentiation/chemistry; Cell Line; Chromatography, Affinity; Dimerization; Epitopes/chemistry; Fatty Acids/antagonists & inhibitors/biosynthesis; Galectin 3; Humans; Hydroxylamine/chemistry; Laminin/metabolism; Protein Precursors/chemistry/metabolism; Receptors, Laminin/biosynthesis/chemistry/metabolism; Solubility

Abstract :

[en] Even though the involvement of the 67-kDa laminin receptor (67LR) in tumor invasiveness has been clearly demonstrated, its molecular structure remains an open problem, since only a full-length gene encoding a 37-kDa precursor protein (37LRP) has been isolated so far. A pool of recently obtained monoclonal antibodies directed against the recombinant 37LRP molecule was used to investigate the processing that leads to the formation of the 67-kDa molecule. In soluble extracts of A431 human carcinoma cells, these reagents recognize the precursor molecule as well as the mature 67LR and a 120-kDa molecule. The recovery of these proteins was found to be strikingly dependent upon the cell solubilization conditions: the 67LR is soluble in NP-40-lysis buffer whereas the 37LRP is NP-40-insoluble. Inhibition of 67LR formation by cerulenin indicates that acylation is involved in the processing of the receptor. It is likely a palmitoylation process, as indicated by sensitivity of NP-40-soluble extracts to hydroxylamine treatment. Immunoblotting assays performed with a polyclonal serum directed against galectin3 showed that both the 67- and the 120-kDa proteins carry galectin3 epitopes whereas the 37LRP does not. These data suggest that the 67LR is a heterodimer stabilized by strong intramolecular hydrophobic interactions, carried by fatty acids bound to the 37LRP and to a galectin3 cross-reacting molecule.

Disciplines :

Oncology
Biochemistry, biophysics & molecular biology

Author, co-author :

Buto, S.

Tagliabue, E.

Ardini, E.

Magnifico, A.

Ghirelli, C.

van den Brule, F.

Castronovo, Vincenzo ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases

Colnaghi, M. I.

Sobel, M. E.

Menard, S.

Language :

English

Title :

Formation of the 67-kDa laminin receptor by acylation of the precursor.

Publication date :

1998

Journal title :

Journal of Cellular Biochemistry

ISSN :

0730-2312

eISSN :

1097-4644

Publisher :

Wiley Liss, Inc.

Volume :

Issue :

Pages :

244-51

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

AIRF/FIRC

Available on ORBi :

since 28 September 2009

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110

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Bibliography

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