In vitro and in vivo biocompatibility of calcium- phosphate scaffolds 3D printed by stereolithography for bone regeneration

Le Guéhennec, Laurent; Van hede, Dorien; Plougonven, Erwan; Nolens, Grégory; Verlée, Bruno; Gillet, Marie-Claire; LAMBERT, France

doi:10.1002/jbm.a.36823

Article (Scientific journals)

In vitro and in vivo biocompatibility of calcium- phosphate scaffolds 3D printed by stereolithography for bone regeneration

Le Guéhennec, Laurent; Van hede, Dorien; Plougonven, Erwan et al.

2019 • In Journal of Biomedical Materials Research. Part A

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/240664

DOI
10.1002/jbm.a.36823

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31654476

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Keywords :

Stereolithography; Bone scaffold; bone regeneration; micro tomography; histology

Abstract :

[en] Stereolithography (SLA) is an interesting manufacturing technology to overcome limitations of commercially available particulated biomaterials dedicated to intra-oral bone regeneration applications. The purpose of this study was to evaluate the in vitro and in vivo biocompatibility and osteoinductive properties of two CaP-based scaffolds manufactured by SLA 3D printing. Pellets and macro-porous scaffolds were manufactured in pure hydroxyapatite (HA) and in biphasic CaP (HA:60-TCP:40). Physico- chemical characterization was performed using Micro X-ray Fluorescence (μXRF), Scanning Electron Microscopy (SEM), optical interferometry and microtomography (μCT) analyses. Osteoblast-like MG-63 cells were used to evaluate the biocompatibility of the pellets in vitro with MTS assay and the cell morphology and growth characterized by SEM and DAPI-actin staining showed similar early behavior. For in vivo biocompatibility, newly formed bone and biodegradability ofthe experimental scaffolds were evaluated in a subperiosteal cranial rat model using μCT and descriptive histology. The histological analysis has not indicated evidences of inflammation but highlighted close contacts between newly formed bone and the experimental biomaterials revealing an excellent scaffold osseointegration. This study emphasizes the relevance of SLA 3D printing of CaP-based biomaterials for intra-oral bone regeneration even if manufacturing accuracy has to be improved and further experiments using biomimetic scaffolds should be conducted.

Research center :

d‐BRU - Dental Biomaterials Research Unit - ULiège [BE]

Disciplines :

Dentistry & oral medicine
Biotechnology

Author, co-author :

Le Guéhennec, Laurent ^✱

Van hede, Dorien ^✱; Université de Liège - ULiège > Département de sciences dentaires > Biomatériaux dentaires

Plougonven, Erwan ; Université de Liège - ULiège > Department of Chemical Engineering > PEPs (Product, Environment, Processes)

Nolens, Grégory

Verlée, Bruno

Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie

LAMBERT, France ; Centre Hospitalier Universitaire de Liège - CHU > Département de dentisterie > Service de médecine dentaire

^✱ These authors have contributed equally to this work.

Language :

English

Title :

In vitro and in vivo biocompatibility of calcium- phosphate scaffolds 3D printed by stereolithography for bone regeneration

Publication date :

October 2019

Journal title :

Journal of Biomedical Materials Research. Part A

ISSN :

1549-3296

eISSN :

1552-4965

Publisher :

John Wiley & Sons, Hoboken, United States - New Jersey

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
FWB - Fédération Wallonie-Bruxelles [BE]

Available on ORBi :

since 28 October 2019

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