Reference : Fifteen shades of green: The evolution of Bufotes toads revisited
Scientific journals : Article
Life sciences : Genetics & genetic processes
Life sciences : Zoology
Fifteen shades of green: The evolution of Bufotes toads revisited
Dufresnes, Christophe [University of Lausanne > > > >]
Mazepa, Glib [University of Lausanne > > > >]
Jablonski, Daniel [Comenius University in Bratislava > > > >]
Oliveira, Ricardo Caliari [KU Leuven > > > >]
Wenseleers, Tom [KU Leuven > > > >]
Shabanov, Dmytro A. [V. N. Karazin Kharkiv National University > > > >]
Auer, Markus [Senckenberg Natural History Collections Dresden > > > >]
Ernst, Raffael [Senckenberg Natural History Collections Dresden > > > >]
Koch, Claudia [Zoologisches Forschungsmuseum Alexander Koenig > > > >]
Ramírez-Chaves, Héctor E. [Universidad de Caldas > > > >]
Mulder, Kevin Patrick [Centro de Investigação em Biodiversidade e Recursos Genéticos da Universidade do Porto > > > >]
Simonov, Evgeniy [Institute of Systematics and Ecology of Animals of the Siberian Branch of the Russian Academy of Sciences > > > >]
Tiutenko, Arthur [University of Erlangen-Nuremberg > > > >]
Kryvokhyzha, Dmytro [Uppsala University > > > >]
Wennekes, Paul L. [University of Reading > > > >]
Zinenko, Oleksandr I. [V. N. Karazin Kharkiv National University > > > >]
Korshunov, Oleksiy V. [V. N. Karazin Kharkiv National University > > > >]
Al-Johany, Awadh M. [King Saud University > > > >]
Peregontsev, Evgeniy A. [Zoocomplex MChJ > > > >]
Masroor, Rafaqat [Pakistan Museum of Natural History > > > >]
Betto-Colliard, Caroline [University of Lausanne > > > >]
Denoël, Mathieu mailto [Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Laboratoire d'Écologie et de Conservation des Amphibiens >]
Borkin, Leo J. [Russian Academy of Sciences > > > >]
Skorinov, Dmitriy V. [Russian Academy of Sciences > > > >]
Pasynkova, Roza A. [Russian Academy of Sciences > > > >]
Mazanaeva, Lyudmila F. [Dagestan State University > > > >]
Rosanov, Juriy M. [Russian Academy of Sciences > > > >]
Dubey, Sylvain [University of Lausanne & Hintermann & Weber > > > >]
Litvinchuk, Spartak [Russian Academy of Sciences > > > >]
Molecular Phylogenetics and Evolution
Yes (verified by ORBi)
[en] Allopolyploidization ; Bufonidae ; Cryptic species ; Green toads ; Hybrid speciation ; RAD sequencing ; Bufotes viridis ; Phylogenomics ; RAD Seq ; Bufotes balearicus ; Bufotes sitibundus ; Bufotes perrini ; Bufotes cypriensis ; Bufotes turanensis ; Bufotes boulengeri ; Bufotes boulengeri siculus ; Bufotes latastii ; Bufotes surdus ; cyto-nuclear discordances ; reproductive isolation ; Phylogeography ; radiation ; Dobzhansky-Muller incompatibilities ; mt-DNA ; Eurasia ; Balkans ; toxin ; call ; map ; admixture ; genetic ; description ; amphibian ; green toad ; holotype
[en] The radiation of Palearctic green toads (Bufotes) holds great potential to evaluate the role of hybridization in phylogeography at multiple stages along the speciation continuum. With fifteen species representing three ploidy levels, this model system is particularly attractive to examine the causes and consequences of allopolyploidization, a prevalent yet enigmatic pathway towards hybrid speciation. Despite substantial efforts, the evolutionary history of this species complex remains largely blurred by the lack of consistency among the corresponding literature. To get a fresh, comprehensive view on Bufotes phylogeography, here we combined genome-wide multilocus analyses (RAD-seq) with an extensive compilation of mitochondrial, genome size, niche modelling, distribution and phenotypic (bioacoustics, morphometrics, toxin composition) datasets, representing hundreds of populations throughout Eurasia. We provide a fully resolved nuclear phylogeny for Bufotes and highlight exceptional cyto-nuclear discordances characteristic of complete mtDNA replacement (in 20% of species), mitochondrial surfing during post-glacial expansions, and the formation of homoploid hybrid populations. Moreover, we traced the origin of several allopolyploids down to species level, showing that all were exclusively fathered by the West Himalayan B. latastii but mothered by several diploid forms inhabiting Central Asian lowlands, an asymmetry consistent with hypotheses on mate choice and Dobzhansky-Muller incompatibilities. Their intermediate call phenotypes potentially allowed for rapid reproductive isolation, while toxin compositions converged towards the ecologically-closest parent. Across the radiation, we pinpoint a stepwise progression of reproductive isolation through time, with a threshold below which hybridizability is irrespective of divergence (<6My), above which species barely admix and eventually evolve different mating calls (6–10My), or can successfully cross-breed through allopolyploidization (>15My). Finally, we clarified the taxonomy of Bufotes (including genetic analyses of type series) and formally described two new species, B. cypriensis sp. nov. (endemic to Cyprus) and B. perrini sp. nov. (endemic to Central Asia). Embracing the genomic age, our framework marks the advent of a new exciting era for evolutionary research in these iconic amphibians.
Freshwater and OCeanic science Unit of reSearch - FOCUS
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Researchers ; Professionals ; Students
This paper is published by Elsevier (see DOI link)

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