Poster (Scientific congresses and symposiums)
NanoLuX: a network-wide Nanoluciferase-based platform to monitor activation of classical and atypical chemokine receptors
Meyrath, Max Marc Roger; Reynders, Nathan; Counson, Manuel et al.
20193rd European Chemokine and Cell Migration Conference (ECMC2019)
 

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Keywords :
chemokine receptors; Nanoluciferase; arrestin
Abstract :
[en] The activity of chemokine receptors is dependent on G proteins that, upon chemokine binding, trigger various intracellular signalling cascades, as well as on b-arrestins that, following receptor phosphorylation by GRK, orchestrate its desensitization, endocytosis and trafficking. In addition to the 19 classical chemokine receptors, 4 receptors form a subfamily of atypical chemokine receptors (ACKR1-4) with ligand scavenging functions (Fig 1). These receptors are unable to couple to G proteins but their activity can be monitored via b-arrestins. In this study we describe the NanoLux platform, a network-wide profiling platform for chemokines and chemokines receptors based on the complementation of the nanoluciferase (NanoBiT). This platform allows to monitor the activation, modulation or bias of receptors or ligands by measuring the binding or the recruitment of effectors, regulators or parners such as G proteins, GRK or b-arrestin isoforms to the classical and the atypical chemokine receptors. For that purpose, we N-terminally fused the LgBiT portion of Nanoluciferase to the MiniGi protein, GRK2 or human b- arrestin 1 and 2, while the SmBiT was fused to the C terminus of all of the 23 human chemokine receptors (Fig 2). Using this approach, we are now able to assess and compare the activity of molecules at the chemokine-receptor network level.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Meyrath, Max Marc Roger ;  Université de Liège - ULiège > Doct. sc. bioméd. & pharma. (paysage)
Reynders, Nathan
Counson, Manuel
Beaupain, Nadia
Ollert, Markus
Szpakowska, Martyna
Chevigne, Andy
Language :
English
Title :
NanoLuX: a network-wide Nanoluciferase-based platform to monitor activation of classical and atypical chemokine receptors
Publication date :
June 2019
Number of pages :
A0
Event name :
3rd European Chemokine and Cell Migration Conference (ECMC2019)
Event place :
Salamanca, Spain
Event date :
26-06-2019 to 30-06-2019
Audience :
International
Funders :
FNR - Fonds National de la Recherche [LU]
Funding text :
Bourse AFR
Available on ORBi :
since 12 July 2019

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