A gel retardation assay system for studying protein binding to simple repetitive DNA sequences.

Maueler, W.; Muller, Marc; Kohne, A. C.; Epplen, J. T.

doi:10.1002/elps.1150130103

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A gel retardation assay system for studying protein binding to simple repetitive DNA sequences.

Maueler, W.; Muller, Marc; Kohne, A. C. et al.

1992 • In Electrophoresis, 13 (1-2), p. 7-10

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/23725

DOI
10.1002/elps.1150130103

PubMed
1587258

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Keywords :

Animals; Base Sequence; Cattle; DNA, Neoplasm/metabolism; DNA, Single-Stranded/genetics; DNA-Binding Proteins/metabolism; Electrophoresis, Polyacrylamide Gel; Humans; Molecular Sequence Data; Protein Binding; Repetitive Sequences, Nucleic Acid/genetics

Abstract :

[en] Simple repetitive DNA sequences have been regarded as mere "junk" present in all eukaryotic genomes. In fact, mixed simple repeat (gt)n(ga)m sequences are present in major histocompatibility complex MHC-DRB genes for long evolutionary times, including such distant animals as artiodactyla and man. We describe herein an unsophisticated method which reveals that at least certain simple repetitive (gt)n(ga)m sequences bind nuclear proteins and show characteristics of a specific DNA-protein interaction via gel retardation.

Disciplines :

Genetics & genetic processes

Author, co-author :

Maueler, W.

Muller, Marc ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Kohne, A. C.

Epplen, J. T.

Language :

English

Title :

A gel retardation assay system for studying protein binding to simple repetitive DNA sequences.

Publication date :

1992

Journal title :

Electrophoresis

ISSN :

0173-0835

eISSN :

1522-2683

Publisher :

Vch Publishers, Weinheim, Germany

Volume :

Issue :

1-2

Pages :

7-10

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 25 September 2009

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Bibliography

Epplen, J.T., Ammer, H., Kammerbauer, C., Schwaiger, W., Schmid, M., Nanda, I., (1991) Adv. Mol. Gen., 4, pp. 301-310
Ammer, H., Schwaiger, F.‐W., Kammerbauer, C., Arriens, A., Lazary, S., Epplen, J.T., (1991), Immunogenetics
Stallings, R.L., Ford, A.F., Nelson, D., Torney, D.C., Hildebrand, C.E., Moyzis, R.K., (1991) Genomics, 10, pp. 807-815
Yee, H.A., Wong, A.K.C., Van de Sande, J.H., Rattner, J.B., (1991) Nucleic Acids Res., 19, pp. 949-953
Joulin, V., Boris, D., Eléouet, J.‐F., Labastie, M.‐C., Chrétien, S., Mattéi, M.‐G., Roméo, P.‐H., (1991) EMBO J., 10, pp. 1809-1816
Wahls, W.P., Swenson, G., Moore, P.D., (1991) Nucleic Acids Res., 19, pp. 3269-3274
Altschmied, J., Muller, M., Baniahmad, A., Steiner, C., Renkawitz, R., (1989) Nucleic Acids Res., 17, pp. 4975-4991
Sedmark, J.J., Großberg, S.E., (1977) Anal. Biochem., 79, pp. 544-552
Baniahmad, A., Steiner, C., Köhne, A.C., Renkawitz, R., (1990) Cell, 61, pp. 505-514
Sambrook, J., Fritsch, E.F., Maniatis, T., (1988) Molecular Cloning: A Laboratory Manual, , Cold Spring Habor Laboratory Press, New York
Rieß, O., Kammerbauer, C., Roewer, L., Steimle, V., Epplen, J.T., (1990) Immunogenetics, 32, pp. 110-116
Schäfer, R., Zischler, H., Birsner, U., Becker, A., Epplen, J.T., (1988) Electrophoresis, 9, pp. 369-374
Epplen, J.T., (1988) J. Hered., 79, pp. 409-417
Buitkamp, J., Kühn, C., Zischler, H., Epplen, J.T., Geldermann, H., (1991) Animal Genet., 22, pp. 137-146