Reference : Cooperative interaction of chicken lysozyme enhancer sub-domains partially overlappin...
Scientific journals : Article
Life sciences : Genetics & genetic processes
Cooperative interaction of chicken lysozyme enhancer sub-domains partially overlapping with a steroid receptor binding site.
Altschmied, J. [> > > >]
Muller, Marc mailto [Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Baniahmad, A. [> > > >]
Steiner, C. [> > > >]
Renkawitz, R. [> > > >]
Nucleic Acids Research
Oxford University Press
Yes (verified by ORBi)
United Kingdom
[en] Animals ; Base Sequence ; Chickens ; Cloning, Molecular ; DNA/genetics/metabolism ; Enhancer Elements, Genetic ; Genes ; Macrophages/metabolism ; Molecular Sequence Data ; Muramidase/genetics ; Nuclear Proteins/metabolism ; Plasmids ; Protein Binding ; Receptors, Glucocorticoid/metabolism ; Receptors, Progesterone/metabolism ; Restriction Mapping
[en] Expression of the lysozyme gene is a marker for the differentiation of macrophages, lysozyme transcription being gradually increased during maturation. We have analyzed the fine structure and function of two macrophage-specific enhancer elements of the chicken lysozyme gene (E-2.7 kb and E-0.2 kb). Both increase their activities upon LPS induction, both contain multiple binding sites for similar or identical nuclear factors and both can be divided into two functional modules. For the E-0.2 kb enhancer we found a synergistic activity of the modules to be dependent on their distance. Binding sites for nuclear proteins within enhancer E-0.2 kb overlap substantially with the previously identified progesterone/glucocorticoid receptor binding site, which is required for steroid induction of lysozyme transcription in the oviduct.

File(s) associated to this reference

Fulltext file(s):

Open access
AltschmiedNar1989.pdfPublisher postprint2.44 MBView/Open

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.