Article (Scientific journals)
Multiple C2 domains and Transmembrane region Proteins (MCTPs) tether membranes at plasmodesmata
Brault, Marie; Petit, Jules; Immel, Françoise et al.
2019In EMBO Reports
Peer Reviewed verified by ORBi
 

Files


Full Text
Brault, Petit et al. revised-text only-final.docx
Author preprint (1.4 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplasmic reticulum (ER) - plasma membrane (PM) contacts coincide with regulation of cell-to-cell signalling. The molecular mechanism and function of membrane tethering within plasmodesmata remains unknown. Here we show that the Multiple C2 domains and Transmembrane region Protein (MCTP) family, key regulators of cell-to-cell signalling in plants, act as ER-PM tethers specifically at plasmodesmata. We report that MCTPs are core plasmodesmata proteins that insert into the ER via their transmembrane region whilst their C2 domains dock to the PM through interaction with anionic phospholipids. A Atmctp3/Atmctp4 loss-of-function mutant induces plant developmental defects while MCTP4 expression in a yeast Δtether mutant partially restores ER-PM tethering. Our data suggest that MCTPs are unique membrane tethers controlling both ER-PM contacts and cell-to-cell signalling.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Brault, Marie
Petit, Jules ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Immel, Françoise
Nicolas, William
Glavier, Marie
Brocard, Lysiane
Gaston, Amélie
Fouche, Mathieu
Hawkins, Timothy
Crowet, Jean-Marc 
Grison, Magali
Germain, Véronique
Rocher, Marion
Kraner, Max
Alva, Vikram
Claverol, Stephane
Paternili, Andrea
Helariutta, Yka
Deleu, Magali  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Lins, Laurence  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Tilsner, Jens
Bayer, Emmanuelle
More authors (12 more) Less
Language :
English
Title :
Multiple C2 domains and Transmembrane region Proteins (MCTPs) tether membranes at plasmodesmata
Publication date :
2019
Journal title :
EMBO Reports
ISSN :
1469-221X
eISSN :
1469-3178
Publisher :
Wiley-Blackwell, United States
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 June 2019

Statistics


Number of views
88 (6 by ULiège)
Number of downloads
2 (1 by ULiège)

Scopus citations®
 
72
Scopus citations®
without self-citations
58
OpenCitations
 
74

Bibliography


Similar publications



Contact ORBi