Reference : At Least Three Subdomains of V-Erba Are Involved in Its Silencing Function
Scientific journals : Article
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/23691
At Least Three Subdomains of V-Erba Are Involved in Its Silencing Function
English
Busch, Kerstin [> > > >]
Martin, Bernd [> >]
Baniahmad, Aria [> > > >]
Renkawitz, Rainer [> > > >]
Muller, Marc mailto [Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire >]
Mar-1997
Molecular Endocrinology
11
3
379-89
Yes (verified by ORBi)
International
0888-8809
[en] v-erbA ; Thyroid hormone receptor ; Repression ; Transcription ; Domain ; Modular
[en] Several members of the thyroid hormone receptor (TR) family are able to switch from a transcriptional repressor to a transcriptional activator upon binding of their ligand. The oncogene v-erbA is a variant form of the TR unable to bind hormone and thus acts as a constitutive repressor. We demonstrate, using fusion proteins between the DNA-binding domain of the yeast factor GAL4 and the silencing domains of v-erbA and TR beta, that point mutations in three different regions severely affect their repression function. Furthermore, the three regions, each as an inactive fusion protein with the GAL4 DNA-binding domain, restore silencing activity when assembled on the same promoter. These observations define at least three silencing subdomains, SSD1-SSD3, which are involved in the silencing function of v-erbA. We propose a model in which full silencing activity is brought about by the combined interaction of each silencing subdomain with corepressors and/or basal transcription factors.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/23691
10.1210/me.11.3.379
http://mend.endojournals.org/cgi/reprint/11/3/379

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