Reference : At Least Three Subdomains of V-Erba Are Involved in Its Silencing Function
Scientific journals : Article
Life sciences : Genetics & genetic processes
At Least Three Subdomains of V-Erba Are Involved in Its Silencing Function
Busch, Kerstin [> > > >]
Martin, Bernd [> >]
Baniahmad, Aria [> > > >]
Renkawitz, Rainer [> > > >]
Muller, Marc mailto [Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire >]
Molecular Endocrinology
Yes (verified by ORBi)
[en] v-erbA ; Thyroid hormone receptor ; Repression ; Transcription ; Domain ; Modular
[en] Several members of the thyroid hormone receptor (TR) family are able to switch from a transcriptional repressor to a transcriptional activator upon binding of their ligand. The oncogene v-erbA is a variant form of the TR unable to bind hormone and thus acts as a constitutive repressor. We demonstrate, using fusion proteins between the DNA-binding domain of the yeast factor GAL4 and the silencing domains of v-erbA and TR beta, that point mutations in three different regions severely affect their repression function. Furthermore, the three regions, each as an inactive fusion protein with the GAL4 DNA-binding domain, restore silencing activity when assembled on the same promoter. These observations define at least three silencing subdomains, SSD1-SSD3, which are involved in the silencing function of v-erbA. We propose a model in which full silencing activity is brought about by the combined interaction of each silencing subdomain with corepressors and/or basal transcription factors.
Researchers ; Professionals ; Students

File(s) associated to this reference

Fulltext file(s):

Restricted access
BuschEtAl1997.pdfNo commentaryPublisher postprint379.96 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.