Article (Scientific journals)
At Least Three Subdomains of V-Erba Are Involved in Its Silencing Function
Busch, Kerstin; Martin, Bernd; Baniahmad, Aria et al.
1997In Molecular Endocrinology, 11 (3), p. 379-89
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Keywords :
v-erbA; Thyroid hormone receptor; Repression; Transcription; Domain; Modular
Abstract :
[en] Several members of the thyroid hormone receptor (TR) family are able to switch from a transcriptional repressor to a transcriptional activator upon binding of their ligand. The oncogene v-erbA is a variant form of the TR unable to bind hormone and thus acts as a constitutive repressor. We demonstrate, using fusion proteins between the DNA-binding domain of the yeast factor GAL4 and the silencing domains of v-erbA and TR beta, that point mutations in three different regions severely affect their repression function. Furthermore, the three regions, each as an inactive fusion protein with the GAL4 DNA-binding domain, restore silencing activity when assembled on the same promoter. These observations define at least three silencing subdomains, SSD1-SSD3, which are involved in the silencing function of v-erbA. We propose a model in which full silencing activity is brought about by the combined interaction of each silencing subdomain with corepressors and/or basal transcription factors.
Disciplines :
Genetics & genetic processes
Author, co-author :
Busch, Kerstin
Martin, Bernd
Baniahmad, Aria
Renkawitz, Rainer
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Language :
English
Title :
At Least Three Subdomains of V-Erba Are Involved in Its Silencing Function
Publication date :
March 1997
Journal title :
Molecular Endocrinology
ISSN :
0888-8809
eISSN :
1944-9917
Publisher :
The Endocrine Society, United States - Maryland
Volume :
11
Issue :
3
Pages :
379-89
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
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