Molecular cloning and characterization of two forms of trout growth hormone cDNA: expression and secretion of tGH-II by Escherichia coli

Rentier-Delrue, Françoise; Swennen, D.; Mercier, L.; Lion, M.; Benrubi, O.; Martial, Joseph

doi:10.1089/dna.1.1989.8.109

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Molecular cloning and characterization of two forms of trout growth hormone cDNA: expression and secretion of tGH-II by Escherichia coli

Rentier-Delrue, Françoise; Swennen, D.; Mercier, L. et al.

1989 • In DNA, 8 (2), p. 109-17

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https://hdl.handle.net/2268/23690

DOI
10.1089/dna.1.1989.8.109

PubMed
2647438

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Keywords :

Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; *Dna; Escherichia coli/metabolism; Growth Hormone/*genetics/secretion; Molecular Sequence Data; Salmon; Salmonidae/*genetics; Sequence Homology, Nucleic Acid; Trout/*genetics

Abstract :

[en] We constructed a cDNA library using mRNA isolated from rainbow trout pituitaries. Two types of cDNA clones encoding growth hormone (GH) were isolated and their complete nucleotide sequences determined. Twenty seven nucleotide substitutions in the coding region and 108 in the noncoding region distinguish the cDNAs of trout GH-I and II. Both cDNAs encode polypeptides of 210 amino acids, including a putative signal peptide of 22 amino acids, which differ by 12 residues. In both trout and salmon, GH-I mRNA is predominant, which suggests that the variation in the amount of secreted GH originates from a transcriptional event. Moreover, comparison of rainbow trout and chum salmon GH reveals that, in both cases, the predominant GH-I has mutated less than its GH-II counterpart. Mature tGH-II was expressed in Escherichia coli using the pIN-III-ompA-Hind secretion vector.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Rentier-Delrue, Françoise ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique

Swennen, D.

Mercier, L.

Lion, M.

Benrubi, O.

Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Language :

English

Title :

Molecular cloning and characterization of two forms of trout growth hormone cDNA: expression and secretion of tGH-II by Escherichia coli

Publication date :

1989

Journal title :

DNA

ISSN :

0198-0238

Publisher :

Mary Ann Liebert, Inc., New York, United States - New York

Volume :

Issue :

Pages :

109-17

Peer reviewed :

Peer Reviewed verified by ORBi

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since 24 September 2009

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