Anoxia; Cyclic electron flow; Cytochrome b6f; Photosynthesis; Photosystem I
Abstract :
[en] Cyclic electron flow (CEF) is defined as a return of the reductants from the acceptor side of Photosystem I (PSI) to the pool of its donors via the cytochrome b6f. It is described to be complementary to the linear electron flow and essential for photosynthesis. However, despite many efforts aimed to characterize CEF, its pathway and its regulation modes remain equivocal, and its physiological significance is still not clear. Here we use novel spectroscopic to measure the rate of CEF at the onset of light in the green alga Chlamydomonas reinhardtii. The initial redox state of the photosynthetic chain or the oxygen concentration do not modify the initial maximal rate of CEF (60 electrons per second per PSI) but rather strongly influence its duration. Neither the maximal rate nor the duration of CEF are different in the pgrl1 mutant compared to the wild type, disqualifying PGRL1 as the ferredoxin-plastoquinone oxidoreductase involved in the CEF mechanism.
Nawrocki, Wojciech J.; Institut de Biologie Physico-Chimique (Paris)
Bailleul, Benjamin; Institut de Biologie Physico-Chimique (Paris)
Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Rappaport, Fabrice; Institut de Biologie Physico-Chimique (Paris)
Wollman, Françis Andrè; Institut de Biologie Physico-Chimique (Paris)
Joliot, Pierre A.; Institut de Biologie Physico-Chimique (Paris)
Language :
English
Title :
Maximal cyclic electron flow rate is independent of PGRL1 in Chlamydomonas.
Publication date :
2019
Journal title :
Biochimica et Biophysica Acta. Bioenergetics
ISSN :
0005-2728
eISSN :
1879-2650
Publisher :
Elsevier, Netherlands
Volume :
1860
Issue :
5
Pages :
425-432
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
H2020 - 682580 - BEAL - Bioenergetics in microalgae : regulation modes of mitochondrial respiration, photosynthesis, and fermentative pathways, and their interactions in secondary algae
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