Chronic exposure to copper and zinc induces DNA damage in the polychaete Alitta virens and the implications for future toxicity of coastal sites

Watson, G. J.; Pini, J. M.; Richir, Jonathan

doi:10.1016/j.envpol.2018.09.091

Article (Scientific journals)

Chronic exposure to copper and zinc induces DNA damage in the polychaete Alitta virens and the implications for future toxicity of coastal sites

Watson, G. J.; Pini, J. M.; Richir, Jonathan

2018 • In Environmental Pollution, p. 1498-1508

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/236319

DOI
10.1016/j.envpol.2018.09.091

PubMed
30293035

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Keywords :

Antifouling; Bioavailability; Genotoxic; Nanoparticles; Nereis virens; Porewater

Abstract :

[en] Copper and zinc are metals that have been traditionally thought of as past contamination legacies. However, their industrial use is still extensive and current applications (e.g. nanoparticles and antifouling paints) have become additional marine environment delivery routes. Determining a pollutant's genotoxicity is an ecotoxicological priority, but in marine benthic systems putative substances responsible for sediment genotoxicity have rarely been identified. Studies that use sediment as the delivery matrix combined with exposures over life-history relevant timescales are also missing for metals. Here we assess copper and zinc's genotoxicity by exposing the ecologically important polychaete Alitta virens to sediment spiked with environmentally relevant concentrations for 9 months. Target bioavailable sediment and subsequent porewater concentrations reflect the global contamination range for coasts, whilst tissue concentrations, although elevated, were comparable with other polychaetes. Survival generally reduced as concentrations increased, but monthly analyses show that growth was not significantly different between treatments. The differential treatment mortality may have enabled the surviving worms in the high concentration treatments to capture more food thus removing any concentration treatment effects for biomass. Using the alkaline comet assay we confirm that both metals via the sediment are genotoxic at concentrations routinely found in coastal regions and this is supported by elevated DNA damage in worms from field sites. However, combined with the growth data it also highlights the tolerance of A. virens to DNA damage. Finally, using long term (decadal) monitoring data we show stable or increasing sediment concentrations of these metals for many areas. This will potentially mean coastal sediment is a significant mutagenic hazard to the benthic community for decades to come. An urgent reappraisal of the current input sources for these ‘old pollutants’ is, therefore, required. Chronic exposure of zinc and copper via sediment at environmentally relevant concentrations induces DNA damage in a marine polychaete. © 2018 Elsevier Ltd

Research Center/Unit :

Institute of Marine Sciences, School of Biological Sciences, University of Portsmouth

Disciplines :

Environmental sciences & ecology

Author, co-author :

Watson, G. J.; Institute of Marine Sciences, School of Biological Sciences, University of Portsmouth, Ferry Road, Portsmouth, United Kingdom

Pini, J. M.; Institute of Marine Sciences, School of Biological Sciences, University of Portsmouth, Ferry Road, Portsmouth, United Kingdom

Richir, Jonathan ; Université de Liège - ULiège > Département d'astrophys., géophysique et océanographie (AGO) > Chemical Oceanography Unit (AGO)

Language :

English

Title :

Chronic exposure to copper and zinc induces DNA damage in the polychaete Alitta virens and the implications for future toxicity of coastal sites

Publication date :

2018

Journal title :

Environmental Pollution

ISSN :

0269-7491

eISSN :

1873-6424

Publisher :

Elsevier Ltd

Pages :

1498-1508

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

part-funded by the European RDF, INTER-REG IVA under part of the CHRONEXPO and 3C projects.

Available on ORBi :

since 04 June 2019

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