Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding.

Adenylate Cyclase Toxin/genetics/metabolism; Amino Acid Substitution; Asparagine/genetics; Bordetella pertussis/metabolism/pathogenicity; CD11b Antigen/chemistry/metabolism; CD18 Antigens/chemistry/metabolism; Glutamine/genetics; Glycosylation; Humans; Macrophage-1 Antigen/genetics/metabolism; Polysaccharides/metabolism; Protein Structure, Tertiary; Adenylate cyclase toxin; CD11b/CD18; Complement receptor type 3; N-linked glycosylation; Point mutants; Repeats in toxin

Abstract :

[en] The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of CR3 were substituted with glutamine residues that cannot be glycosylated. Examination of CR3 mutant variants and mass spectrometry analysis of the N-glycosylation pattern of CR3 revealed that N-glycans located in the C-terminal part of the CD11b subunit are involved in binding and cytotoxic activity of CyaA. We suggest that these N-glycans form a defined clustered saccharide patch that enables multivalent contact of CR3 with CyaA, enhancing both affinity and specificity of the integrin-toxin interaction.

Disciplines :

Microbiology

Author, co-author :

Hasan, Shakir ; Université de Liège - ULiège > I3-Immunophysiology

Osickova, Adriana

Bumba, Ladislav

Novak, Petr

Sebo, Peter

Osicka, Radim

Language :

English

Title :

Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding.

Publication date :

2015

Journal title :

FEBS Letters

ISSN :

0014-5793

eISSN :

1873-3468

Publisher :

Wiley-Blackwell, United States

Volume :

589

Issue :

Pages :

374-9

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 28 May 2019

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