A guide to polarized airway epithelial models for studies of host-pathogen interactions.

Transwells; air-liquid interface; airway epithelium; bacterial pathogenesis; barrier function; host-pathogen interactions; innate immunity; in vitro models; polarized airway epithelial cells

Abstract :

[en] Mammalian lungs are organs exhibiting the cellular and spatial complexity required for gas exchange to support life. The respiratory epithelium internally lining the airways is susceptible to infections due to constant exposure to inhaled microbes. Biomedical research into respiratory bacterial infections in humans has been mostly carried out using small mammalian animal models or two-dimensional, submerged cultures of undifferentiated epithelial cells. These experimental model systems have considerable limitations due to host specificity of bacterial pathogens and lack of cellular and morphological complexity. This review describes the in vitro differentiated and polarized airway epithelial cells of human origin that are used as a model to study respiratory bacterial infections. Overall, these models recapitulate key aspects of the complexity observed in vivo and can help in elucidating the molecular details of disease processes observed during respiratory bacterial infections.

Disciplines :

Microbiology

Author, co-author :

Hasan, Shakir ; Université de Liège - ULiège > I3-Immunophysiology

Sebo, Peter

Osicka, Radim

Language :

English

Title :

A guide to polarized airway epithelial models for studies of host-pathogen interactions.

Publication date :

2018

Journal title :

FEBS Journal

ISSN :

1742-464X

eISSN :

1742-4658

Publisher :

Blackwell, Oxford, United Kingdom

Volume :

285

Issue :

Pages :

4343-4358

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 28 May 2019

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