Nano-on-micro fibrous extracellular matrices for scalable expansion of human ES/iPS cells.

Batch Cell Culture Techniques/methods; Biomimetic Materials/chemistry; Cell Differentiation/physiology; Cell Proliferation/physiology; Cells, Cultured; Extracellular Matrix/chemistry; Human Embryonic Stem Cells/cytology/physiology; Humans; Nanofibers/chemistry/ultrastructure; Pluripotent Stem Cells/cytology/physiology; Tissue Engineering/instrumentation/methods; Tissue Scaffolds; Extracellular matrix; Human pluripotent stem cells; Nanofiber; Scaled-up culture; Self-renewal

Abstract :

[en] Human pluripotent stem cells (hPSCs) hold great potential for industrial and clinical applications. Clinical-grade scaffolds and high-quality hPSCs are required for cell expansion as well as easy handling and manipulation of the products. Current hPSC culture methods do not fulfill these requirements because of a lack of proper extracellular matrices (ECMs) and cell culture wares. We developed a layered nano-on-micro fibrous cellular matrix mimicking ECM, named "fiber-on-fiber (FF)" matrix, which enables easy handling and manipulation of cultured cells. While non-woven sheets of cellulose and polyglycolic acid were used as a microfiber layer facilitating mechanical stability, electrospun gelatin nanofibers were crosslinked on the microfiber layer, generating a mesh structure with connected nanofibers facilitating cell adhesion and growth. Our results showed that the FF matrix supports effective hPSC culture with maintenance of their pluripotency and normal chromosomes over two months, as well as effective scaled-up expansion, with fold increases of 54.1 +/- 15.6 and 40.4 +/- 8.4 in cell number per week for H1 human embryonic stem cells and 253G1 human induced pluripotent stem cells, respectively. This simple approach to mimick the ECM may have important implications after further optimization to generate lineage-specific products.

Disciplines :

Biotechnology

Author, co-author :

Liu, Li

Kamei, Ken-Ichiro

Yoshioka, Momoko

Nakajima, Minako

Li, Junjun

Fujimoto, Nanae

Terada, Shiho

Tokunaga, Yumie ; Université de Liège - ULiège > Medical Genomics-Unit of Animal Genomics

Koyama, Yoshie

Sato, Hideki

More authors (3 more)

Language :

English

Title :

Nano-on-micro fibrous extracellular matrices for scalable expansion of human ES/iPS cells.

Publication date :

2017

Journal title :

Biomaterials

ISSN :

0142-9612

eISSN :

1878-5905

Publisher :

Elsevier, United Kingdom

Volume :

124

Pages :

47-54

Peer reviewed :

Peer Reviewed verified by ORBi

Commentary :

Available on ORBi :

since 22 May 2019

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Bibliography

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