Sub1 contacts the RNA polymerase II stalk to modulate mRNA synthesis

[en] Biogenesis of messenger RNA is critically influenced by the phosphorylation state of the carboxy-terminal domain (CTD) in the largest RNA polymerase II (RNAPII) subunit. Several kinases and phosphatases are required to maintain proper CTD phosphorylation levels and, additionally, several other proteins modulate them, including Rpb4/7 and Sub1. The Rpb4/7 heterodimer, constituting the RNAPII stalk, promote phosphatase functions and Sub1 globally influences CTD phosphorylation, though its mechanism remains mostly unknown. Here, we show that Sub1 physically interacts with the RNAPII stalk do- main, Rpb4/7, likely through its C-terminal region, and associates with Fcp1. While Rpb4 is not required for Sub1 interaction with RNAPII complex, a fully functional heterodimer is required for Sub1 association to promoters. We also demonstrate that a complete CTD is necessary for proper association of Sub1 to chromatin and to the RNAPII. Finally, genetic data show a functional relationship between Sub1 and the RNAPII clamp domain. Altogether, our results indicate that Sub1, Rpb4/7 and Fcp1 interaction modulates CTD phosphorylation. In addition, Sub1 interaction with Rpb4/7 can also modulate transcription start site selection and transcription elongation rate likely by influencing the clamp function.

Disciplines :

Genetics & genetic processes

Author, co-author :

Garavis, Miguel

Gonzalez Polo, Noelia

Allepuz Fuster, Paula ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Alegrio Louro, Jaime

Fernandez Tornero, Carlos

Calvo, Olga

Language :

English

Title :

Sub1 contacts the RNA polymerase II stalk to modulate mRNA synthesis

Publication date :

2017

Journal title :

Nucleic Acids Research

ISSN :

0305-1048

eISSN :

1362-4962

Publisher :

Oxford University Press, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 10 May 2019

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Bibliography

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