Splenic microRNA expression profiles and integration analyses involved in host responses to Salmonella enteritidis infection in chickens

Li, P.; Fan, W.; Li, Q.; Wang, J.; Liu, R.; Everaert, Nadia; Liu, Jian; Zhang, Y.; Zheng, M.; Cui, H.; Zhao, Guoying; Wen, J.

doi:10.3389/fcimb.2017.00377

Article (Scientific journals)

Splenic microRNA expression profiles and integration analyses involved in host responses to Salmonella enteritidis infection in chickens

Li, P.; Fan, W.; Li, Q. et al.

2017 • In Frontiers in Cellular and Infection Microbiology, 7 (AUG)

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/235273

DOI
10.3389/fcimb.2017.00377

PubMed
28884089

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Keywords :

Chicken; Clinical symptoms; MicroRNA; MiRNA-target genes; Next generation sequencing; Salmonella enteritidis; Spleen; Salmonella enteritidis vaccine; IRF4 protein, Gallus gallus; LRRC59 protein, human; Article; Leghorn chicken; RNA extraction; Salmonella enterica serovar Enteritidis; Adaptive Immunity; Animals; Apoptosis; Avian Proteins; Cell Line; Chickens; Host-Pathogen Interactions; Humans; Interferon Regulatory Factors; Membrane Proteins; MicroRNAs; NLR Proteins; Poultry Diseases; RNA, Messenger; Salmonella Infections, Animal; Transcriptome

Abstract :

[en] To understand the role of miRNAs in regulating genes involved in the host response to Salmonella enteritidis (SE) infection, next generation sequencing was applied to explore the altered splenic expression of microRNAs (miRNAs) and deregulated genes in specific-pathogen-free chickens. Birds were either infected or not (controls, C) and those challenged with SE were evaluated 24 h later and separated into two groups on the basis of the severity of clinical symptoms and blood load of SE: resistant (R, SE challenged-slight clinical symptoms and <10 5 cfu / 10 μL), and susceptible (S, SE challenged-severe clinical symptoms and >10 7 cfu/10 μL). Thirty-two differentially expressed (DE) miRNAs were identified in spleen, including 16 miRNAs between S and C, 13 between R and C, and 13 between S and R. Through integration analysis of DE miRNAs and mRNA, a total of 273 miRNA-target genes were identified. Functional annotation analysis showed that Apoptosis and NOD-like receptor signaling pathway and adaptive immune response were significantly enriched (P < 0.05). Interestingly, apoptosis pathway was significantly enriched in S vs. C, while NOD-like receptor pathway was enriched in R vs. C (P < 0.05). Two miRNAs, gga-miR-101-3p and gga-miR-155, in the hub positions of the miRNA-mRNA regulatory network, were identified as candidates potentially associated with SE infection. These 2 miRNAs directly repressed luciferase reporter gene activity via binding to 3'-untranslated regions of immune-related genes IRF4 and LRRC59; over-expressed gga-miR-155 and interference gga-miR-101-3p in chicken HD11 macrophage cells significantly altered expression of their target genes and decreased the production of pro-inflammatory cytokines. These findings facilitate better understanding of the mechanisms of host resistance and susceptibility to SE infection in chickens. © 2017 Li, Fan, Li, Wang, Liu, Everaert, Liu, Zhang, Zheng, Cui, Zhao and Wen.

Disciplines :

Animal production & animal husbandry

Author, co-author :

Li, P.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China, Precision Livestock and Nutrition Unit, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium, State Key Laboratory of Animal Nutrition, Beijing, China

Fan, W.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China

Li, Q.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China

Wang, J.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China, State Key Laboratory of Animal Nutrition, Beijing, China

Liu, R.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China

Everaert, Nadia ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions animales et nutrition

Liu, Jian

Zhang, Y.; College of Animal Science, Jilin University, Changchun, China

Zheng, M.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China

Cui, H.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China

Zhao, Guoying ; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, C

Wen, J.; Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China, State Key Laboratory of Animal Nutrition, Beijing, China

Language :

English

Title :

Splenic microRNA expression profiles and integration analyses involved in host responses to Salmonella enteritidis infection in chickens

Publication date :

2017

Journal title :

Frontiers in Cellular and Infection Microbiology

eISSN :

2235-2988

Publisher :

Frontiers Media S.A.

Volume :

Issue :

AUG

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 08 May 2019

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