Heterologous production of NRPs: From the identification of the biosynthesis genes to the productive heterologous yeast

Non-ribosomal peptides (NRPs) represent a huge group of microbial secondary metabolites with broad and potent biological activities such as antibiotics, antifungal, anti-inflammatory, immunosuppressive, surfactant, etc. Therefore these natural products could find applications in
numerous fields (pharmaceutic , cosmetology, or agronomic by means of biological control agents) . Numerous NRP producer organisms are
still unknown and understudied despite the promising activities of the NRPs they synthesize. Moreover, some of these organisms are not easily
cultivable or have not immediate applicability to industrial processes. Therefore, to gain suitable production rates of these compounds of interest, one option consists in working with a heterologous production using more efficient strains. Based on the example of a non-ribosomal pentapeptide produced initially by a filamentous fungus, we elaborated a general methodology to develop an eukaryotic microbial platform in a yeast for the heterologous production of NRPs.