dopamine neuron; action potential; substantia nigra
Abstract :
[en] In many neuronal types, axon initial segment (AIS) geometry critically influences neuronal excitability. Interestingly, the axon of rat substantia nigra pars compacta (SNc) dopaminergic (DA) neurons displays a highly variable location and most often arises from an axon-bearing dendrite (ABD). We combined current-clamp somatic and dendritic recordings, outside-out recordings of dendritic sodium and potassium currents, morphological reconstructions and multi-compartment modelling on male and female rat SNc DA neurons to determine cell-to-cell variations in AIS and ABD geometry and their influence on neuronal output (spontaneous pacemaking frequency, AP shape). Both AIS and ABD geometries were found to be highly variable from neuron to neuron. Surprisingly, we found that AP shape and pacemaking frequency were independent of AIS geometry. Modelling realistic morphological and biophysical variations clarify this result: in SNc DA neurons, the complexity of the ABD combined with its excitability predominantly define pacemaking frequency and AP shape, such that large variations in AIS geometry negligibly affect neuronal output, and are tolerated.
Research center :
Giga-Neurosciences - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Moubarak, Estelle
Engel, Dominique ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Pharmacologie
Dufour, Martial
Tapia, Monica
Tell, Fabien
Goaillard, Jean-Marc
Language :
English
Title :
Robustness to axon initial segment variation is explained by somatodendritic excitability in rat substantia nigra dopaminergic neurons
Publication date :
26 June 2019
Journal title :
Journal of Neuroscience
ISSN :
0270-6474
eISSN :
1529-2401
Publisher :
Society for Neuroscience, United States
Volume :
39
Issue :
26
Pages :
5044-5063
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
FP7 - 616827 - CANALOHMICS - Biophysical networks underlying the robustness of neuronal excitability
Funders :
ANR - Agence Nationale de la Recherche [FR] CE - Commission Européenne [BE]
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