Study of the lipid profile of ATCC and clinical strains of staphylococcus aureus in relation to their antibiotic resistance

Bisignano, C.; Ginestra, G.; Smeriglio, A.; La Camera, E.; Crisafi, G.; Franchina, Flavio; Tranchida, P. Q.; Alibrandi, A.; Trombetta, D.; Mondello, L.; Mandalari, G.

doi:10.3390/molecules24071276

Article (Scientific journals)

Study of the lipid profile of ATCC and clinical strains of staphylococcus aureus in relation to their antibiotic resistance

Bisignano, C.; Ginestra, G.; Smeriglio, A. et al.

2019 • In Molecules, 24 (7)

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/234731

DOI
10.3390/molecules24071276

PubMed
30986911

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Keywords :

Antibiotic resistance; ATCC strains; Clinical strains; Hydrophobicity; Lipids; Staphylococcus aureus

Abstract :

[en] A number of reports have indicated a relationship between bacterial resistance to antibiotics and their lipid composition. In the present study, we characterized the lipid profiles of American Type Culture Collection (ATCC) and clinical strains of Staphylococcus aureus and its correlation with antibiotic resistance and hydrophobicity. The following strains were used: S. aureus ATCC 6538P, S. aureus ATCC 43300 (MRSA), seven clinical strains from the pharynges, two strains from duodenal ulcers, four strains from hip prostheses, and one strain from the conjunctiva. Lipid-related differentiation was observed across the S. aureus strains: the higher abundance of anteiso-pentadecanoic acid (anteiso-C 15:0 ) and anteiso-heptadecanoic acid (anteiso-C 17:0 ), followed by iso-pentadecanoic acid (iso-C 15:0 ), suggested that these were common lipids. Iso-tridecanoic acid (iso-C 13:0 ) and anteiso-tridecanoic acid (anteiso-C 13:0 ), iso-hexadecanoic acid (iso-C 16:0 ) and anteiso-hexadecanoic acid (anteiso-C 16:0 ), and all forms of octadecanoic acid (C 18:0 ) were usually detected in low abundance. Strains isolated from pharynges showed the highest ratio of branched/straight chains. A distinction in two clusters based on the amount and type of bacterial lipids identified was obtained, which correlated to the antibiotic resistance, the strains origin, and the cell-surface hydrophobicity. We report a potential correlation between the lipid profile of S. aureus strains, site of infection, antibiotic resistance, and cell-surface hydrophobicity. These results, which still need further insights, could be a first step to identifying antibiotic resistance in response to environmental adaptation. © 2019 by the authors.

Disciplines :

Life sciences: Multidisciplinary, general & others

Author, co-author :

Bisignano, C.; Department of Biomedical, Dental, Morphological and Functional Images Sciences, University of Messina, Via C. Valeria, Messina, 98125, Italy

Ginestra, G.; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, I-98168, Italy

Smeriglio, A.; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, I-98168, Italy

La Camera, E.; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, I-98168, Italy

Crisafi, G.; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, I-98168, Italy

Franchina, Flavio ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique

Tranchida, P. Q.; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, I-98168, Italy

Alibrandi, A.; Department of Economics, Unit of Statistical and Mathematical Sciences, University of Messina, Messina, 98125, Italy

Trombetta, D.; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, I-98168, Italy

Mondello, L.; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, I-98168, Italy, Chromaleont c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, I-98168, Italy

Mandalari, G.; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, I-98168, Italy

Language :

English

Title :

Study of the lipid profile of ATCC and clinical strains of staphylococcus aureus in relation to their antibiotic resistance

Publication date :

2019

Journal title :

Molecules

eISSN :

1420-3049

Publisher :

MDPI AG

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 24 April 2019

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