Article (Scientific journals)
First attempts for vitrification of immature oocytes in donkey (Equus asinus): Comparison of two vitrification methods
Douet, Cécile; Reigner, Fabrice; Barrière, P. et al.
2019In Theriogenology, 126, p. 261-265
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Keywords :
Immature; Jenny; Oocyte; Ovum pick up; Vitrification
Abstract :
[en] Most wild donkey breeds are severely threatened by poaching for meat, habitat loss, and competition with livestock for food resources. Moreover, due to the mechanization in agriculture and in transport, most domestic donkey breeds are at risk of extinction. Considering the importance of biodiversity and preservation of genetic resources, the creation of genetic banks for endangered donkey breeds is urgently needed. Cryopreservation of immature jennies oocytes would be an efficient tool to allow storage of female genetics. The aim of the present study was to establish conditions for immature donkey oocyte vitrification, using equine oocytes as a control. Asine and equine immature cumulus-oocyte complexes were collected by transvaginal ultrasound-guided follicular aspiration and flushed to obtain oocytes surrounded by only corona radiata. Oocytes were vitrified after exposure to increasing concentrations of dimethyl sulfoxide, ethylene glycol and sucrose as cryoprotectants in a solution of INRA-Freeze™ medium or TCM199-Hepes supplemented with bovine serum albumin. Oocytes were warmed in decreasing concentrations of sucrose and processed for in vitro maturation. The recovery rate was 48% for jennies oocytes (4.8 oocyte per female) and 42% for mares oocytes (3.5 oocyte per female). When oocytes were exposed to cryoprotectants in INRA-Freeze™ medium none of the jennies re-warmed oocytes matured, whereas 24% of the mares re-warmed oocytes reached metaphase II after in vitro maturation. When oocytes were exposed to cryoprotectants in TCM199-Hepes-BSA medium, 33% of the jennies re-warmed oocytes matured. In conclusion, we developed a method for the vitrification of immature oocytes from jennies that allows in vitro maturation of the vitrified-warmed asine oocytes. Their competence for fertilization and development has to be ascertain. © 2018 Elsevier Inc.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Douet, Cécile;  PRC, INRA, CNRS, IFCE, Université de Tours, Nouzilly, 37380, France
Reigner, Fabrice;  UE1297 PAO, INRA, Nouzilly, 37380, France
Barrière, P.;  UE1297 PAO, INRA, Nouzilly, 37380, France
Blard, T.;  UE1297 PAO, INRA, Nouzilly, 37380, France
Deleuze, Stefan  ;  Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Obstét. et path. de la reprod. des anim. de comp. et équidés
Goudet, Ghylene;  PRC, INRA, CNRS, IFCE, Université de Tours, Nouzilly, 37380, France
Language :
English
Title :
First attempts for vitrification of immature oocytes in donkey (Equus asinus): Comparison of two vitrification methods
Publication date :
2019
Journal title :
Theriogenology
ISSN :
0093-691X
eISSN :
1879-3231
Publisher :
Elsevier Inc.
Volume :
126
Pages :
261-265
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 February 2019

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