Identification of new bioactive peptides from Kefir milk through proteopeptidomics: Bioprospection of antihypertensive molecules

Amorim, F. G.; Coitinho, L. B.; Dias, A. T.; Friques, A. G. F.; Monteiro, B. L.; Rezende, L. C. D. D.; Pereira, T. D. M. C.; Campagnaro, B. P.; De Pauw, Edwin; Vasquez, E. C.; Quinton, Loïc

doi:10.1016/j.foodchem.2019.01.010

Article (Scientific journals)

Identification of new bioactive peptides from Kefir milk through proteopeptidomics: Bioprospection of antihypertensive molecules

Amorim, F. G.; Coitinho, L. B.; Dias, A. T. et al.

2019 • In Food Chemistry, 282, p. 109-119

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/233228

DOI
10.1016/j.foodchem.2019.01.010

PubMed
30711094

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Keywords :

ACE; Antihypertension; Bioactive peptides; Inhibition; Kefir; Proteomics; Beverages; Enzyme activity; Fermented milk; Mammals; Mass spectrometry; Molecular biology; Molecules; Peptides; Product design; Angiotensin-converting enzyme; Anti-hypertension; Anti-hypertensive activities; MALDI-TOF mass spectrometry; Systolic arterial pressures; Enzyme inhibition

Abstract :

[en] Kefir, a probiotic beverage prepared from fermented milk, has been associated with antihypertensive activity. However, the bioactive molecules responsible for this activity still remain unclear. Therefore, in this study we aim to evaluate the mechanisms of the antihypertensive effects of Kefir in the two-kidney one-clip hypertension model, and to bioprospect for bioactive peptides identified by proteomic methodologies. Treatment with Kefir was performed via gavage, and resulted in a 37 mmHg reduction in systolic arterial pressure and 19% inhibition of angiotensin converting enzyme (ACE) activity. For the proteopeptidomic study, the protein extract of Kefir beverage and non-fermented bovine milk were analysed by MALDI-TOF mass spectrometry, and their tryptic digestion products sequenced via Shotgun proteomics (Q-Exactive mass spectrometer). A list of 35 peptides with potential hypertensive activity due to ACE inhibition were identified. These results demonstrate the benefits of Kefir products, and may guide the design of new antihypertensive drugs. © 2019 Elsevier Ltd

Disciplines :

Chemistry

Author, co-author :

Amorim, F. G.; Pharmaceutical Sciences Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

Coitinho, L. B.; School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo – USP, Ribeirão Preto, SP, Brazil

Dias, A. T.; Laboratory of Translational Physiology, Federal University of Espirito Santo, Vitoria, ES, Brazil

Friques, A. G. F.; Pharmaceutical Sciences Graduate Program, Vila Velha University, Vila Velha, ES, Brazil, Laboratory of Translational Physiology, Federal University of Espirito Santo, Vitoria, ES, Brazil

Monteiro, B. L.; Laboratory of Translational Physiology, Federal University of Espirito Santo, Vitoria, ES, Brazil

Rezende, L. C. D. D.; Departament of Natural Science, Federal University of Espirito Santo, CEUNES, São Mateus, ES, Brazil

Pereira, T. D. M. C.; Pharmaceutical Sciences Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

Campagnaro, B. P.; Pharmaceutical Sciences Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique

Vasquez, E. C.; Pharmaceutical Sciences Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

Quinton, Loïc ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie biologique

Language :

English

Title :

Identification of new bioactive peptides from Kefir milk through proteopeptidomics: Bioprospection of antihypertensive molecules

Publication date :

2019

Journal title :

Food Chemistry

ISSN :

0308-8146

eISSN :

1873-7072

Publisher :

Elsevier Ltd

Volume :

282

Pages :

109-119

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 February 2019

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