Unpublished conference/Abstract (Scientific congresses and symposiums)
FINE-TUNING POLYMER-BASED BIOMONITORING TOOLS FOR QUICK AND COST-EFFECTIVE SCREENING OF PERSISTENT, BIOACCUMULATIVE AND TOXIC CONTAMINANTS (PBTs) IN LIPID CONTAINING MATRICES
Doan Thi Que
201719th International Symposium on Pollutant Responses in Marine Organisms
 

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Keywords :
persistent organic pollutants; biomonitoring; mixture toxicity
Abstract :
[en] Quantifying the exposure of biota to persistent, bioaccumulative and toxic compounds (PBTs) is time-consuming and costly, and traditionally requires chemically targeted extraction and cleanup. New polymer-based passive samplers with the potential to overcome these limitations have been recently developed. This study aimed to evaluate whether fine-tuning of the polymers can enhance their performance as biomonitoring tools (i.e. high sorptive capacity and low lipid swelling, which can cause errors or require further cleanup). A set of methacrylate-based polymers were developed using graft polymerization on polydimethylsiloxane (PDMS). We investigated whether increased degrees of grafting enhanced sorptive capacity and whether cross-linking of the grafts reduced swelling by lipids. Batch experiments were used to quantify Klipid-polymer and Clipid (µglipid/mgpolymer) using PCDDs and PCBs as test PBTs. Poly-butyl methacrylate (PBMA) showed the highest sorptive capacity, which increased with increasing monomer concentrations used in the grafting process (e.g. when the concentration of butyl methacrylate monomer increased from 1 M to 4 M, Klipid-polymer decreased from 2.43 ± 0.61 to 1.58 ± 0.04, reflecting a 1.5-fold increase in the sorptive capacity). Higher sorptive capacity was generally associated with an increasing mass of lipid transferred into polymers. However, equilibrium concentrations of lipid in PBMA could be considerably reduced by increasing the cross-linking degree, possibly due to the associated decrease in the mesh size of the polymer network causing exclusion of large (70-100 Å) storage lipids. Tighter cross-linking did not affect the chemical sorptive capacity of the polymer grafts. The sorptive capacity of PBMA prepared with 4 M of monomer and 15% (feeding ratio) of cross-linker was consistent and sufficiently high to detect PBTs at typical background levels in biota tissue (1-1000pg PBTs/g lipid). Overall, the study demonstrated that fine-tuned PBMA-based samplers may offer a simple, quick and cost-effective tool for biomonitoring of PBT mixtures in lipid containing matrices.
Research center :
National Research Centre for Environmental Toxicology (Entox), The University of Queensland
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Doan Thi Que ;  Université de Liège - ULiège > Form. doct. sc. (bioch., biol. mol. cel., bioinf. - paysage)
Language :
English
Title :
FINE-TUNING POLYMER-BASED BIOMONITORING TOOLS FOR QUICK AND COST-EFFECTIVE SCREENING OF PERSISTENT, BIOACCUMULATIVE AND TOXIC CONTAMINANTS (PBTs) IN LIPID CONTAINING MATRICES
Publication date :
June 2017
Event name :
19th International Symposium on Pollutant Responses in Marine Organisms
Event place :
Japan
Event date :
30/06/2017 to 03/07/2017
Audience :
International
Available on ORBi :
since 12 February 2019

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