Article (Scientific journals)
Detection of intraocular Leptospira spp. by real‐time polymerase chain reaction in horses with recurrent uveitis in Belgium
Sauvage, Aurélie; Monclin, Sébastien; Elansary, Mahmoud et al.
2019In Equine Veterinary Journal
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Keywords :
horse; ERU; Leptospira spp.; aqueous humour; vitreous humour; PCR
Abstract :
[en] Background Equine recurrent uveitis (ERU) has been associated with Leptospira spp. infection. No information exists concerning the prevalence of Leptospira‐associated ERU in Belgium and about the sensitivity of detection of Leptospira in different ocular media. Objectives To establish the prevalence of intraocular Leptospira spp. in ERU‐affected and healthy eyes of horses examined at the Equine Clinic of the University of Liège by real‐time PCR and to compare the results of the aqueous and vitreous humour of the same eye. Study design Cross‐sectional. Methods Sixty‐six eyes from 59 client‐owned horses with a diagnosis of equine recurrent uveitis (ERU‐group) were studied from May 2015 to December 2017. Fifty healthy eyes from 28 euthanised horses for unrelated reasons examined during the same period were included in the control group. Intraocular fluids (aqueous and/or vitreous humours) from ERU‐affected eyes were sampled and analysed by real‐time PCR for Leptospira spp. Aqueous and vitreous humours from the control group were processed in the same way. Results Both groups were comparable regarding age, sex, eye sampled (OS/OD), humours sampled (aqueous/vitreous humour) but not regarding breeds, with an over‐representation of Warmbloods and Appaloosas in the ERU‐group. The prevalence of Leptospira spp. was 30.3% (20/66 eyes) in the ERU‐group. Leptospira spp. DNA was identified in 11 aqueous and 17 vitreous humours with eight horses testing positive in both humours, nine horses testing positive for vitreous humour alone and third horses for aqueous humour alone. The phi‐correlation between aqueous and vitreous humour Leptospira‐PCR results is 0.47 suggesting a low association. All the control eyes were negative. Main limitations The diagnostic method selected for this study (lipL32 qPCR) did not allow identification of the serovars. Conclusions Leptospirosis is a potential cause of ERU in Belgium. Testing both intraocular media is advised whenever possible.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Sauvage, Aurélie ;  Université de Liège - ULiège > Clinique vétérinaire universitaire (CVU)
Monclin, Sébastien ;  Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Pathologie médicale des petits animaux
Elansary, Mahmoud ;  Université de Liège - ULiège > Unit of Animal Genomics > GIGA‐R and College of Veterinary Medicine
Hansen, Pascale;  Synlab > Vétérinaire
Grauwels, Magda ;  Université de Liège - ULiège > CVU : pôle des animaux de compagnie
Language :
English
Title :
Detection of intraocular Leptospira spp. by real‐time polymerase chain reaction in horses with recurrent uveitis in Belgium
Publication date :
2019
Journal title :
Equine Veterinary Journal
ISSN :
0425-1644
eISSN :
2042-3306
Publisher :
Equine Veterinary Journal, United Kingdom
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 10 February 2019

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