Detection of intraocular Leptospira spp. by real‐time polymerase chain reaction in horses with recurrent uveitis in Belgium

Sauvage, Aurélie; Monclin, Sébastien; Elansary, Mahmoud; Hansen, Pascale; Grauwels, Magda

doi:10.1111/evj.13012

Article (Scientific journals)

Detection of intraocular Leptospira spp. by real‐time polymerase chain reaction in horses with recurrent uveitis in Belgium

Sauvage, Aurélie; Monclin, Sébastien; Elansary, Mahmoud et al.

2019 • In Equine Veterinary Journal

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/232794

DOI
10.1111/evj.13012

PubMed
30144314

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Keywords :

horse; ERU; Leptospira spp.; aqueous humour; vitreous humour; PCR

Abstract :

[en] Background Equine recurrent uveitis (ERU) has been associated with Leptospira spp. infection. No information exists concerning the prevalence of Leptospira‐associated ERU in Belgium and about the sensitivity of detection of Leptospira in different ocular media. Objectives To establish the prevalence of intraocular Leptospira spp. in ERU‐affected and healthy eyes of horses examined at the Equine Clinic of the University of Liège by real‐time PCR and to compare the results of the aqueous and vitreous humour of the same eye. Study design Cross‐sectional. Methods Sixty‐six eyes from 59 client‐owned horses with a diagnosis of equine recurrent uveitis (ERU‐group) were studied from May 2015 to December 2017. Fifty healthy eyes from 28 euthanised horses for unrelated reasons examined during the same period were included in the control group. Intraocular fluids (aqueous and/or vitreous humours) from ERU‐affected eyes were sampled and analysed by real‐time PCR for Leptospira spp. Aqueous and vitreous humours from the control group were processed in the same way. Results Both groups were comparable regarding age, sex, eye sampled (OS/OD), humours sampled (aqueous/vitreous humour) but not regarding breeds, with an over‐representation of Warmbloods and Appaloosas in the ERU‐group. The prevalence of Leptospira spp. was 30.3% (20/66 eyes) in the ERU‐group. Leptospira spp. DNA was identified in 11 aqueous and 17 vitreous humours with eight horses testing positive in both humours, nine horses testing positive for vitreous humour alone and third horses for aqueous humour alone. The phi‐correlation between aqueous and vitreous humour Leptospira‐PCR results is 0.47 suggesting a low association. All the control eyes were negative. Main limitations The diagnostic method selected for this study (lipL32 qPCR) did not allow identification of the serovars. Conclusions Leptospirosis is a potential cause of ERU in Belgium. Testing both intraocular media is advised whenever possible.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Sauvage, Aurélie ; Université de Liège - ULiège > Clinique vétérinaire universitaire (CVU)

Monclin, Sébastien ; Université de Liège - ULiège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Pathologie médicale des petits animaux

Elansary, Mahmoud ; Université de Liège - ULiège > Unit of Animal Genomics > GIGA‐R and College of Veterinary Medicine

Hansen, Pascale; Synlab > Vétérinaire

Grauwels, Magda ; Université de Liège - ULiège > CVU : pôle des animaux de compagnie

Language :

English

Title :

Detection of intraocular Leptospira spp. by real‐time polymerase chain reaction in horses with recurrent uveitis in Belgium

Publication date :

2019

Journal title :

Equine Veterinary Journal

ISSN :

0425-1644

eISSN :

2042-3306

Publisher :

Equine Veterinary Journal, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 10 February 2019

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Bibliography

Gerding, J.C. and Gilger, B.C. (2016) Prognosis and impact of equine recurrent uveitis. Equine Vet. J. 48, 290-298.
Gilger, B.C. and Hollingsworth, S.R. (2017) Diseases of the uvea, uveitis, and recurrent uveitis. In: Equine Ophthalmology, 3rd edn., Ed: B.C. Gilger, John Wiley & Sons, Ames, Iowa. pp 376-422.
Romeike, A., Brügmann, M. and Drommer, W. (1998) Immunohistochemical studies in equine recurrent uveitis (ERU). Vet. Pathol. 35, 515-526.
Gilger, B.C., Malok, E., Cutter, K.V., Stewart, T., Horohov, D.W. and Allen, J.B. (1999) Characterization of T-lymphocytes in the anterior uvea of eyes with chronic equine recurrent uveitis. Vet. Immunol. Immunopathol. 71, 17-28.
Deeg, C.A., Ehrenhofer, M., Thurau, S.R., Reese, S., Wildner, G. and Kaspers, B. (2002) Immunopathology of recurrent uveitis in spontaneously diseased horses. Exp. Eye Res. 75, 127-133.
Regan, D.P., Aarnio, M.C., Davis, W.S., Carmichael, K.P., Vandenplas, M.L., Lauderdale, J.D. and Moore, P.A. (2012) Characterization of cytokines associated with Th17 cells in the eyes of horses with recurrent uveitis. Vet. Ophthalmol. 15, 145-152.
Malalana, F., Stylianides, A. and McGowan, C. (2015) Equine recurrent uveitis: human and equine perspectives. Vet. J. 206, 22-29.
Witkowski, L., Cywinska, A., Paschalis-Trela, K., Crisman, M. and Kita, J. (2016) Multiple etiologies of equine recurrent uveitis – a natural model for human autoimmune: a brief review. Comp. Immunol. Microbiol. Infect. Dis. 44, 14-20.
Simeonova, G.P., Krastev, S.Z. and Simeonov, R.S. (2016) Immunological and pathological investigations in equine experimental uveitis. Vet. Res. Commun. 40, 107-115.
Faber, N.A., Crawford, M., Lefebvre, R.B., Buyukmihci, N.C., Madigan, J.E. and Willits, N.H. (2000) Detection of Leptospira spp. in the aqueous humor of horses with naturally acquired recurrent uveitis. J. Clin. Microbiol. 38, 2731-2733.
Polle, F., Storey, E., Eades, S., Alt, D., Hornsby, R., Zuerner, R. and Carter, R. (2014) Role of intraocular leptospira infections in the pathogenesis of equine recurrent uveitis in the Southern United States. J. Equine. Vet. Sci. 34, 1300-1306.
Malalana, F., Blundell, R.J., Pinchbeck, G.L. and Mcgowan, C.M. (2017) The role of Leptospira spp. in horses affected with recurrent uveitis in the UK. Equine Vet. J. 49, 706-709.
Gilger, B.C., Salmon, J.H., Yi, N.Y., Barden, C.A., Chandler, H.L., Wendt, J.A. and Colitz, C.M. (2008) Role of bacteria in the pathogenesis of recurrent uveitis in horses from the southeastern United States. Am. J. Vet. Res. 69, 1329-1335.
Brandes, K., Wollanke, B., Niedermaier, G., Brem, S. and Gerhards, H. (2007) Recurrent uveitis in horses: vitreal examinations with ultrastructural detection of leptospires. J. Vet. Med. A. Physiol. Pathol. Clin. Med. 54, 270-275.
Curto, E., Messengerb, K.M., Salmona, J.H. and Gilger, B.C. (2016) Cytokine and chemokine profiles of aqueous humor and serum in horses with uveitis measured using multiplex bead immunoassay analysis. Vet. Immunol. Immunopathol. 182, 43-51.
Pearce, J.W., Galle, L.E., Kleiboeker, S.B., Turk, J.R., Schommer, S.K., Dubielizig, R.R., Mitchell, W.J., Moore, C.P. and Giuliano, E.A. (2007) Detection of Leptospira interrogans DNA and antigen in fixed equine eyes affected with end-stage equine recurrent uveitis. J. Vet. Diagn. Invest. 19, 686-690.
Tömördy, E., Hässig, M. and Spiess, B.M. (2010) The outcome of pars plana vitrectomy in horses with equine recurrent uveitis with regard to the presence or absence of intravitreal antibodies against various serovars of Leptospira interrogans. Pferdeheilkunde 26, 251-254.
Dorrego-Keiter, E., Tóth, J., Dikker, L., Sielhorst, J. and Schusser, G.F. (2016) Detection of leptospira by culture of vitreous humor and detection of antibodies against leptospira in vitreous humor and serum of 225 horses with equine recurrent uveitis. Berl. Munch. Tierarztl. Wochenschr. 129, 209-215.
Spiess, B.M. (2010) Equine recurrent uveitis: the European viewpoint. Equine Vet. J. 42, Suppl. 37, 50-56.
Gilger, B.C. (2010) Equine recurrent uveitis: the viewpoint from the USA. Equine Vet. J. 42, Suppl. 37, 57-61.
Allbaugh, R.A. (2017) Equine recurrent uveitis: a review of clinical assessment and management. Equine Vet. Educ. 29, 279-288.
Gilger, B.C., Wilkie, D.A., Clode, A.B., McMullen, R.J. Jr, Utter, M.E., Komaromy, A.M., Brooks, D.E. and Salmon, J.H. (2010) Long-term outcome after implantation of a suprachoroidal cyclosporine drug delivery device in horses with recurrent uveitis. Vet. Ophthalmol. 13, 294-300.
Erol, E., Jackson, C.B., Steinman, M., Meares, K., Donahoe, J., Kelly, N., Locke, S., Smith, J.L. and Carter, C.N. (2015) A diagnostic evaluation of real-time PCR, fluorescent antibody and microscopic agglutination tests in cases of equine leptospiral abortion. Equine Vet. J. 47, 171-174.
Musso, D. and La Scola, B. (2013) Laboratory diagnosis of leptospirosis: a challenge. J. Microbiol. Immunol. Infect. 46, 245-252.
Verma, A., Stevenson, B. and Adler, B. (2013) Leptospirosis in horses. Vet. Microbiol. 167, 61-66.
Fonseca Cde, A., Teixeira, M.M., Romero, E.C., Tengan, F.M., Silva, M.V. and Shikanai-Yasuda, M.A. (2006) Leptospira DNA detection for the diagnosis of human leptospirosis. J. Infect. 52, 15-22.
Dos Santos, F.F., Commodaro, A.G., Souza, A.V., Pinho, J.R., Sitnik, R., Garcia, C., Ribeiro, A.L., Muccioli, C., Lottenberg, C.L., Rizzo, L.V. and Belfort Junior, R. (2011) Real-time PCR in infectious uveitis as an alternative diagnosis. Arq. Bras. Oftalmol. 74, 258-261.
Slack, A.T., Symonds, M.L., Dohnt, M.F. and Smythe, L.D. (2006) Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene. BMC Microbiol. 6, 95.
Stoddard, R.A., Gee, J.E., Wilkins, P.P., McCaustland, K. and Hoffmaster, A.R. (2009) Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene. Diagn. Microbiol. Infect. Dis. 64, 247-255.
Bourhy, P., Bremont, S., Zinini, F., Giry, C. and Picardeau, M. (2011) Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences. J. Clin. Microbiol. 49, 2154-2160.
De Groot-Mijnes, J.D., Rothova, A., Van Loon, A.M., Schuller, M., Ten Dam-Van Loon, N.H., De Boer, J.H., Schuurman, R. and Weersink, A.J. (2006) Polymerase chain reaction and Goldmann-Witmer coefficient analysis are complimentary for the diagnosis of infectious uveitis. Am. J. Ophthalmol. 141, 313-318.
Matos, K., Muccioli, C., Belfort Junior, R. and Rizzo, L.V. (2007) Correlation between clinical diagnosis and PCR analysis of serum, aqueous, and vitreous samples in patients with inflammatory eye disease. Arq. Bras. Oftalmol. 70, 109-114.
Stoppini, R. and Gilger, B.C. (2017) Equine ocular examination basic techniques. In: Equine Ophthalmology, 3rd edn., Ed: B.C. Gilger, John Wiley & Sons, Ames, Iowa. pp 1-39.
Dreier, J., Störmer, M. and Kleesiek, K. (2005) Use of Bacteriophage MS2 as an internal control in viral reverse transcription-PCR assays. J. Clin. Microbiol. 43, 4551-4557.
R Core Team. (2015) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria. Available at: https://www.R-project.org/ (Accessed July 19 2017).
Robert-Gangneux, F., Binisti, P., Antonetti, D., Brezin, A., Yera, H. and Dupouy-Camet, J. (2004) Usefulness of immunoblotting and Goldmann-Witmer coefficient for biological diagnosis of toxoplasmic retinochoroiditis. Eur. J. Clin. Microbiol. Infect. Dis. 23, 34-38.
Fritz, K.L., Kaese, H.J., Valberg, S.J., Hendrickson, J.A., Rendahl, A.K., Bellone, R.R., Dynes, K.M., Wagner, M.L., Lucio, M.A., Cuomo, F.M., Brinkmeyer-Langford, C.L., Skow, L.C., Mickelson, J.R., Rutherford, M.S. and McCue, M.E. (2014) Genetic risk factors for insidious equine recurrent uveitis in Appaloosa horses. Anim. Genet. 45, 392-399.
Deeg, C.A., Marti, E., Gaillard, C. and Kaspers, B. (2004) Equine recurrent uveitis is strongly associated with the MHC class 1 haplotype ELA-A9. Equine Vet. J. 36, 73-75.
Kulbrock, M., Lehner, S., Metzger, J., Ohnesorge, B. and Distl, O. (2013) A genome-wide association study identifies risk loci to equine recurrent uveitis in German warmblood horses. PLoS One 8, e71619. https://doi.org/10.1371/journal.pone.0071619. eCollection.
Taylor, A.J., Paris, D.H. and Newton, P.N. (2015) A systematic review of the mortality from untreated leptospirosis. PLoS Negl. Trop Dis. 9, e0003866. https://doi.org/10.1371/journal.pntd.0003866. eCollection 2015.
Wynwood, S.J., Graham, G.C., Weier, S.L., Collet, T.A., McKay, D.B. and Craig, S.B. (2014) Leptospirosis from water sources. Pathog. Glob. Health 108, 334-338.
Dupouey, J., Faucher, B., Edouard, S., Richet, H., Kodjo, A., Drancourt, M. and Davoust, B. (2014) Review: human leptospirosis: an emerging risk in Europe? Comp. Immunol. Microbiol. Infect. Dis. 37, 77-83.
Pijnacker, R., Goris, M.G., Te Wierik, M.J., Broens, E.M., van der Giessen, J.W., de Rosa, M., Wagenaar, J.A., Hartskeerl, R.A., Notermans, D.W., Maassen, K. and Schimmer, B. (2016) Marked increase in leptospirosis infections in humans and dogs in the Netherlands, 2014. Euro. Surveill. 28, 21. https://doi.org/10.2807/15607917. ES.2016.21.17.30211.
White, A.M., Zambrana-Torrelio, C., Allen, T., Rostal, M.K., Wright, A.K., Ball, E.C., Daszak, P. and Karesh, W.B. (2017) Hotspots of canine leptospirosis in the United States of America. Vet. J. 222, 29-35.
Delaude, A., Rodriguez-Campos, S., Dreyfus, A., Counotte, M.J., Francey, T., Schweighauser, A., Lettry, S. and Schuller, S. (2017) Canine leptospirosis in Switzerland – a prospective cross-sectional study examining seroprevalence, risk factors and urinary shedding of pathogenic leptospires. Prev. Vet. Med. 141, 48-60.