[en] Quantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for
the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (OIE) reference laboratories and three collaborating laboratories to measure the interlaboratory variability of six already developed and available BLV qPCR assays. For that purpose, an international panel of 58 DNA samples reflecting the dynamic range of the majority of the assays was distributed to six testing centers. Based on qualitative results, the overall agreement among all six laboratories was moderate. However, significant variability in the measurement of the BLV proviral DNA copy number was observed among different laboratories. Quantitative PCR assays, even when performed by experienced staff, can yield large variability in BLV proviral DNA copy numbers without harmonization. Further standardization of different factors (i.e., utilization of unified protocols and unique calibrators) should increase interlaboratory agreement.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Jaworski, J. P.
Pluta, A.
Rola-Łuszczak, M.
McGowan, S. L.
Finnegan, C.
Heenemann, K.
Carignano, H. A.
Alvarez, I.
Murakami, K.
Willems, Luc ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Ghysdael J, Bruck C, Kettmann R, Burny A. 1984. Bovine leukemia virus. Curr Top Microbiol Immunol 112:1-19.
Gillet N, Florins A, Boxus M, Burteau C, Nigro A, Vandermeers F, Balon H, Bouzar AB, Defoiche J, Burny A, Reichert M, Kettmann R, Willems L. 2007. Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human. Retrovirology 4:18. Https://doi.org/10.1186/1742-4690-4-18.
Frie MC, Coussens PM. 2015. Bovine leukemia virus: a major silent threat to proper immune responses in cattle. Vet Immunol Immunopathol 163:103-114. Https://doi.org/10.1016/j.vetimm.2014.11.014.
OIE. 2012. Manual of diagnostic tests and vaccines for terrestrial animals, Chapter 2.4.11, p 721-732. OIE, Paris, France.
Heenemann K, Lapp S, Teifke JP, Fichtner D, Mettenleiter TC, Vahlenkamp TW. 2012. Development of a bovine leukemia virus polymerase gene-based real-time polymerase chain reaction and comparison with an envelope gene-based assay. J Vet Diagn Invest 24:649-655. Https://doi.org/10.1177/1040638712447524.
Lew AE, Bock RE, Molloy JB, Minchin CM, Robinson SJ, Steer P. 2004. Sensitive and specific detection of proviral bovine leukemia virus by 5= Taq nuclease PCR using a 3= minor groove binder fluorogenic probe. J Virol Methods 115:167-175. Https://doi.org/10.1016/j.jviromet.2003.09 .029.
Rola-Luszczak M, Finnegan C, Olech M, Choudhury B, Kuzmak J. 2013. Development of an improved real time PCR for the detection of bovine leukaemia provirus nucleic acid and its use in the clarification of inconclusive serological test results. J Virol Methods 189:258-264. Https://doi.org/10.1016/j.jviromet.2013.02.014.
Juliarena MA, Gutierrez SE, Ceriani C. 2007. Determination of proviral load in bovine leukemia virus-infected cattle with and without lymphocytosis. Am J Vet Res 68:1220-1225. Https://doi.org/10.2460/ajvr.68.11 .1220.
Takeshima SN, Sasaki S, Meripet P, Sugimoto Y, Aida Y. 2017. Single nucleotide polymorphisms in the bovine MHC region of Japanese Black cattle are associated with bovine leukemia virus proviral load. Retrovirology 14:24. Https://doi.org/10.1186/s12977-017-0348-3.
Lorenz RJ, Straub OC. 1994. The problem of recurrence of enzootic bovine leukosis in previously cured herds. Dtsch Tierarztl Wochenschr 101:158-162. (In German.)
Martin D, Arjona A, Soto I, Barquero N, Viana M, Gomez-Lucia E. 2001. Comparative study of PCR as a direct assay and ELISA and AGID as indirect assays for the detection of bovine leukaemia virus. J Vet Med B Infect Dis Vet Public Health 48:97-106. Https://doi.org/10.1111/j.1439-0450.2001.00424.x.
Debacq C, Sanchez Alcaraz MT, Mortreux F, Kerkhofs P, Kettmann R, Willems L. 2004. Reduced proviral loads during primo-infection of sheep by bovine leukemia virus attenuated mutants. Retrovirology 1:31. Https://doi.org/10.1186/1742-4690-1-31.
Kuckleburg CJ, Chase CC, Nelson EA, Marras SA, Dammen MA, Christopher-Hennings J. 2003. Detection of bovine leukemia virus in blood and milk by nested and real-time polymerase chain reactions. J Vet Diagn Invest 15: 72-76. Https://doi.org/10.1177/104063870301500117.
Le QT, Zhang Q, Cao H, Cheng AJ, Pinsky BA, Hong RL, Chang JT, Wang CW, Tsao KC, Lo YD, Lee N, Ang KK, Chan AT, Chan KC. 2013. An international collaboration to harmonize the quantitative plasma Epstein-Barr virus DNA assay for future biomarker-guided trials in nasopharyngeal carcinoma. Clin Cancer Res 19:2208-2215. Https://doi.org/10.1158/1078-0432.CCR-12-3702.
Robardet E, Picard-Meyer E, Andrieu S, Servat A, Cliquet F. 2011. International interlaboratory trials on rabies diagnosis: an overview of results and variation in reference diagnosis techniques (fluorescent antibody test, rabies tissue culture infection test, mouse inoculation test) and molecular biology techniques. J Virol Methods 177:15-25. Https://doi.org/10.1016/j.jviromet.2011.06.004.
Jones RM, Hertwig S, Pitman J, Vipond R, Aspan A, Bolske G, McCaughey C, McKenna JP, van Rotterdam BJ, de Bruin A, Ruuls R, Buijs R, Roest HJ, Sawyer J. 2011. Interlaboratory comparison of real-time polymerase chain reaction methods to detect Coxiella burnetii, the causative agent of Q fever. J Vet Diagn Invest 23:108-111. Https://doi.org/10.1177/104063871102300118.
Muyldermans G, Debaisieux L, Fransen K, Marissens D, Miller K, Vaira D, Vandamme AM, Vandenbroucke AT, Verhofstede C, Schuurman R, Zissis G, Lauwers S. 2000. Blinded, multicenter quality control study for the quantification of human immunodeficiency virus type 1 RNA in plasma by the Belgian AIDS reference laboratories. Clin Microbiol Infect 6:213-217. Https://doi.org/10.1046/j.1469-0691.2000.00048.x.
Somura Y, Sugiyama E, Fujikawa H, Murakami K. 2014. Comparison of the copy numbers of bovine leukemia virus in the lymph nodes of cattle with enzootic bovine leukosis and cattle with latent infection. Arch Virol 159:2693-2697. Https://doi.org/10.1007/s00705-014-2137-9.
Meade KG, Gormley E, Doyle MB, Fitzsimons T, O'Farrelly C, Costello E, Keane J, Zhao Y, MacHugh DE. 2007. Innate gene repression associated with Mycobacterium bovis infection in cattle: toward a gene signature of disease. BMC Genomics 8:400. Https://doi.org/10.1186/1471-2164-8-400.
Gillet NA, Hamaidia M, de Brogniez A, Gutierrez G, Renotte N, Reichert M, Trono K, Willems L. 2016. Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo. PLoS Pathog 12:e1005588. Https://doi.org/10.1371/journal.ppat.1005588.
Katoh K, Standley DM. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772-780. Https://doi.org/10.1093/molbev/mst010.
Lefort V, Longueville JE, Gascuel O. 2017. SMS: smart model selection in PhyML. Mol Biol Evol 34:2422-2424. Https://doi.org/10.1093/molbev/msx149.
Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59: 307-321. Https://doi.org/10.1093/sysbio/syq010.
Kumar S, Stecher G, Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33: 1870-1874. Https://doi.org/10.1093/molbev/msw054.
Humphreys I, Fleming V, Fabris P, Parker J, Schulenberg B, Brown A, Demetriou C, Gaudieri S, Pfafferott K, Lucas M, Collier J, Huang KH, Pybus OG, Klenerman P, Barnes E. 2009. Full-length characterization of hepatitis C virus subtype 3a reveals novel hypervariable regions under positive selection during acute infection. J Virol 83:11456-11466. Https://doi.org/10.1128/JVI.00884-09.
Mirsky ML, Olmstead CA, Da Y, Lewin HA. 1996. The prevalence of proviral bovine leukemia virus in peripheral blood mononuclear cells at two subclinical stages of infection. J Virol 70:2178-2183.
Eaves FW, Molloy JB, Dimmock CK, Eaves LE. 1994. A field evaluation of the polymerase chain reaction procedure for the detection of bovine leukaemia virus proviral DNA in cattle. Vet Microbiol 39:313-321. Https://doi.org/10.1016/0378-1135(94)90167-8.
Juliarena MA, Barrios CN, Ceriani MC, Esteban EN. 2016. Bovine leukemia virus (BLV)-infected cows with low proviral load are not a source of infection for BLV-free cattle. J Dairy Sci 99:4586-4589. Https://doi.org/10.3168/jds.2015-10480.
Yuan Y, Kitamura-Muramatsu Y, Saito S, Ishizaki H, Nakano M, Haga S, Matoba K, Ohno A, Murakami H, Takeshima SN, Aida Y. 2015. Detection of the BLV provirus from nasal secretion and saliva samples using BLV-CoCoMo-qPCR-2: comparison with blood samples from the same cattle. Virus Res 210:248-254. Https://doi.org/10.1016/j.virusres.2015.08 .013.
McHugh ML. 2012. Interrater reliability: the kappa statistic. Biochem Med (Zagreb) 22:276-282. Https://doi.org/10.11613/BM.2012.031.
Dube S, Bachman S, Spicer T, Love J, Choi D, Esteban E, Ferrer JF, Poiesz BJ. 1997. Degenerate and specific PCR assays for the detection of bovine leukaemia virus and primate T cell leukaemia/lymphoma virus pol DNA and RNA: phylogenetic comparisons of amplified sequences from cattle and primates from around the world. J Gen Virol 78(Part 6):1389-1398. Https://doi.org/10.1099/0022-1317-78-6-1389.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.