Reference : Retention of immune complexes by murine lymph node or spleen follicular dendritic cel...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Retention of immune complexes by murine lymph node or spleen follicular dendritic cells. Role of antibody isotype.
Heinen, Ernst mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine >]
Coulie, P. [> > > >]
Van Snick, J. [> > > >]
Braun, M. [> > > >]
Cormann, N. [> > > >]
Moeremans, M. [> > > >]
Kinet-Denoel, C. [> > > >]
Simar, L. J. [> > > >]
Scandinavian Journal of Immunology
Blackwell Publishing
Yes (verified by ORBi)
United Kingdom
[en] Animals ; Antibodies/classification/immunology ; Antigen-Antibody Complex/metabolism ; Antigen-Presenting Cells/immunology ; Complement Fixation Tests ; Immunoglobulin G/immunology ; Immunoglobulin M/immunology ; Lymph Nodes/immunology ; Mice ; Spleen/immunology
[en] Using monoclonal anti-trinitrophenyl (TNP) antibodies complexed to TNP-myoglobin-coated gold particles, we analysed at the ultrastructural level the retention by follicular dendritic cells (FDC) of immune complexes containing various antibody isotypes. Gold-labelled immune complexes were injected subcutaneously or intravenously into naive mice and, after 24 h, germinal centres of draining lymph nodes or spleen were examined by electron microscopy. FDC generally retained complexes containing IgG2a and IgG2b better than those formed with IgG1 or IgG3. IgM was rarely retained. FDC isolated from lymph nodes or spleens were incubated in vitro with gold-labelled complexes in a serum-free medium. IgG2a and IgG2b complexes were also retained in vitro in large quantities by FDC; IgG1 and IgG3 complexes were retained in smaller quantities or in highly variable quantities compared with IgG2; IgM complexes were rarely seen on FDC. There was no difference between FDC isolated from lymph nodes or from spleen with respect to the Ig isotypes required for Fc-mediated retention of immune complexes.

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