Manufacturing Mesenchymal Stromal Cells for the Treatment of Graft-versus-Host Disease: A Survey among Centers Affiliated with the European Society for Blood and Marrow Transplantation

Trento, C.; Bernardo, M. E.; Nagler, A.; Kuçi, S.; Bornhäuser, M.; Köhl, U.; Strunk, D.; Galleu, A.; Sanchez-Guijo, F.; Gaipa, G.; Introna, M.; Bukauskas, A.; Le Blanc, K.; Apperley, J.; Roelofs, H.; Van Campenhout, A.; Beguin, Yves; Kuball, J.; Lazzari, L.; Avanzini, M. A.; Fibbe, W.; Chabannon, C.; Bonini, C.; Dazzi, F.

doi:10.1016/j.bbmt.2018.07.015

Article (Scientific journals)

Manufacturing Mesenchymal Stromal Cells for the Treatment of Graft-versus-Host Disease: A Survey among Centers Affiliated with the European Society for Blood and Marrow Transplantation

Trento, C.; Bernardo, M. E.; Nagler, A. et al.

2018 • In Biology of Blood and Marrow Transplantation, 24, p. 2365-2370

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/232301

DOI
10.1016/j.bbmt.2018.07.015

PubMed
30031938

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Keywords :

Cellular therapy; Graft-versus-host disease; Manufacturing; Mesenchymal stromal cells; Product specification; Release criteria

Abstract :

[en] The immunosuppressive properties of mesenchymal stromal cells (MSC) have been successfully tested to control clinical severe graft-versus host disease and improve survival. However, clinical studies have not yet provided conclusive evidence of their efficacy largely because of lack of patients’ stratification criteria. The heterogeneity of MSC preparations is also a major contributing factor, as manufacturing of therapeutic MSC is performed according to different protocols among different centers. Understanding the variability of the manufacturing protocol would allow a better comparison of the results obtained in the clinical setting among different centers. In order to acquire information on MSC manufacturing we sent a questionnaire to the European Society for Blood and Marrow Transplantation centers registered as producing MSC. Data from 17 centers were obtained and analyzed by means of a 2-phase questionnaire specifically focused on product manufacturing. Gathered information included MSC tissue sources, MSC donor matching, medium additives for ex vivo expansion, and data on MSC product specification for clinical release. The majority of centers manufactured MSC from bone marrow (88%), whilst only 2 centers produced MSC from umbilical cord blood or cord tissue. One of the major changes in the manufacturing process has been the replacement of fetal bovine serum with human platelet lysate as medium supplement. 59% of centers used only third-party MSC, whilst only 1 center manufactured exclusively autologous MSC. The large majority of these facilities (71%) administered MSC exclusively from frozen batches. Aside from variations in the culture method, we found large heterogeneity also regarding product specification, particularly in the markers used for phenotypical characterization and their threshold of expression, use of potency assays to test MSC functionality, and karyotyping. The initial data collected from this survey highlight the variability in MSC manufacturing as clinical products and the need for harmonization. Until more informative potency assays become available, a more homogeneous approach to cell production may at least reduce variability in clinical trials and improve interpretation of results. © 2018

Disciplines :

Hematology

Author, co-author :

Trento, C.; School of Cancer & Pharmaceutical Sciences, King's College London, London, United Kingdom, United Kingdom

Bernardo, M. E.; Pediatric Immunohematology and Bone Marrow Transplantation Unit, San Raffaele Telethon Institute for Gene Therapy (TIGET), San Raffaele Scientific Institute, Milan, Italy, Italy

Nagler, A.; Hematology Division, Chaim Sheba Medical Center, Tel Aviv University, Tel-Hashomer, Israel

Kuçi, S.; Division for Stem Cell Transplantation and Immunology, Frankfurt am Main, Germany

Bornhäuser, M.; Medizinische Klinik und Poliklinik I, Dresden, Germany

Köhl, U.; Institute of Clinical Immunology, University Leipzig, Leipzig, Germany, Fraunhofer Institute of Cellular Therapy and Immunology and Institute of Cellular Therapeutics, Hannover Medical School, Hannover, Germany

Strunk, D.; Experimental & Clinical Cell Therapy Institute, Spinal Cord Injury and Tissue Regeneration Center, Paracelsus Medical University, Salzburg, Austria

Galleu, A.; School of Cancer & Pharmaceutical Sciences, King's College London, London, United Kingdom, United Kingdom

Sanchez-Guijo, F.; Hematology Department, IBSAL-Hospital Universitario de Salamanca, University of Salamanca, Salamanca, Spain

Gaipa, G.; Laboratorio di Terapia Cellulare e Genica Stefano Verri, ASST Monza, Monza, Italy

More authors (14 more)

Language :

English

Title :

Manufacturing Mesenchymal Stromal Cells for the Treatment of Graft-versus-Host Disease: A Survey among Centers Affiliated with the European Society for Blood and Marrow Transplantation

Publication date :

2018

Journal title :

Biology of Blood and Marrow Transplantation

ISSN :

1083-8791

eISSN :

1523-6536

Publisher :

Elsevier Inc.

Volume :

Pages :

2365-2370

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

AIRC - Associazione Italiana per la Ricerca sul Cancro
GSK - GlaxoSmithKline

Available on ORBi :

since 29 January 2019

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Bibliography

Dominici, M, Le Blanc, K, Mueller, I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8 (2006), 315–317.
Bianco, P, Cao, X, Frenette, PS, et al. The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine. Nat Med 19 (2013), 35–42.
Lv, F-J, Tuan, RS, Cheung, KMC, Leung, VYL, Concise review: the surface markers and identity of human mesenchymal stem cells. Stem Cells 32 (2014), 1408–1419.
Crisan, M, Yap, S, Casteilla, L, et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell 3 (2008), 301–313.
Kalluri, R, Zeisberg, M, Fibroblasts in cancer. Nat Rev Cancer 6 (2006), 392–401.
Krampera, M, Glennie, S, Dyson, J, et al. Bone marrow mesenchymal stem cells inhibit the response of naive and memory antigen-specific T cells to their cognate peptide. Blood 101 (2003), 3722–3729.
Bernardo, ME, Fibbe, WE, Mesenchymal stromal cells: sensors and switchers of inflammation. Cell Stem Cell 13 (2013), 392–402.
Cheung, TS, Dazzi, F, Mesenchymal-myeloid interaction in the regulation of immunity. Semin Immunol 35 (2018), 59–68.
Ciccocioppo, R, Bernardo, ME, Sgarella, A, et al. Autologous bone marrow-derived mesenchymal stromal cells in the treatment of fistulising Crohn's disease. Gut 60 (2011), 788–798.
Perin, EC, Sanz-Ruiz, R, Sánchez, PL, et al. Adipose-derived regenerative cells in patients with ischemic cardiomyopathy: the PRECISE Trial. Am Heart J, 168, 2014 88-95.e2.
Laroye, C, Gibot, S, Reppel, L, Bensoussan, D, Concise review: mesenchymal stromal/stem cells: a new treatment for sepsis and septic shock. Stem Cells 35 (2017), 2331–2339.
Lazarus, HM, Koc, ON, Devine, SM, et al. Cotransplantation of HLA-identical sibling culture-expanded mesenchymal stem cells and hematopoietic stem cells in hematologic malignancy patients. Biol Blood Marrow Transplant 11 (2005), 389–398.
de Lima, M, McNiece, I, Robinson, SN, et al. Cord-blood engraftment with ex vivo mesenchymal-cell coculture. N Engl J Med 367 (2012), 2305–2315.
Trento, C, Marigo, I, Pievani, A, et al. Bone marrow mesenchymal stromal cells induce nitric oxide synthase-dependent differentiation of CD11b+ cells that expedite hematopoietic recovery. Haematologica 102 (2017), 818–825.
Le Blanc, K, Frassoni, F, Ball, L, et al. Mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host disease: a phase II study. Lancet 371 (2008), 1579–1586.
Ball, LM, Bernardo, ME, Roelofs, H, et al. Multiple infusions of mesenchymal stromal cells induce sustained remission in children with steroid-refractory, grade III-IV acute graft-versus-host disease. Br J Haematol 163 (2013), 501–509.
von Dalowski, F, Kramer, M, Wermke, M, et al. Mesenchymal stromal cells for treatment of acute steroid-refractory graft versus host disease: clinical responses and long-term outcome. Stem Cells 34 (2016), 357–366.
Sánchez-Guijo, F, Caballero-Velázquez, T, López-Villar, O, et al. Sequential third-party mesenchymal stromal cell therapy for refractory acute graft-versus-host disease. Biol Blood Marrow Transplant 20 (2014), 1580–1585.
Le Blanc, K, Rasmusson, I, Sundberg, B, et al. Treatment of severe acute graft-versus-host disease with third party haploidentical mesenchymal stem cells. Lancet 363 (2004), 1439–1441.
Introna, M, Lucchini, G, Dander, E, et al. Treatment of graft versus host disease with mesenchymal stromal cells: a phase I study on 40 adult and pediatric patients. Biol Blood Marrow Transplant 20 (2014), 375–381.
Galleu, A, Deplano, S, Szydlo, R, et al. Mesenchymal stromal cells for the treatment of steroid-resistant acute graft versus host disease: factors influencing clinical responses. Haematologica 102:suppl 2 (2017), 336–337.
de Girolamo, L, Lucarelli, E, Alessandri, G, et al. Mesenchymal stem/stromal cells: a new “cells as drugs’’ paradigm. Efficacy and critical aspects in cell therapy. Curr Pharm Des 19 (2013), 2459–2473.
Galleu, A, Riffo-Vasquez, Y, Trento, C, et al. Apoptosis in mesenchymal stromal cells induces in vivo recipient-mediated immunomodulation. Sci Transl Med, 2017 9:eaam7828.
Kuci, Z, Bonig, H, Kreyenberg, H, et al. Mesenchymal stromal cells from pooled mononuclear cells of multiple bone marrow donors as rescue therapy in pediatric severe steroid-refractory graft-versus-host disease: a multicenter survey. Haematologica 101 (2016), 985–994.
Avanzini, MA, Bernardo, ME, Cometa, AM, et al. Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord blood- and bone marrow-derived progenitors. Haematologica 94 (2009), 1649–1660.
Vogel, G, Cell biology. To scientists’ dismay, mixed-up cell lines strike again. Science, 329, 2010, 1004.
Torsvik, A, Røsland, GV, Svendsen, A, et al. Spontaneous malignant transformation of human mesenchymal stem cells reflects cross-contamination: putting the research field on track—letter. Cancer Res 70 (2010), 6393–6396.