24,25-Dihydroxyvitamin D; 25-Hydroxyvitamin D; 25-OHD(3):24,25(OH)(2)D(3) ratio; DEQAS; Hypercalcemia; National Institute of Standards and Technology (NIST); Vitamin D Standardization Program (VDSP)
Abstract :
[en] The discovery that mutations of the CYP24A1 gene are a cause of idiopathic infantile hypercalcemia (IIH) has revived interest in measuring serum 24,25(OH)2D3. Several studies have also suggested that a high 25-hydroxyvitamin D3(25-OHD3):24,25(OH)2D3 ratio might provide additional diagnostic information in the investigation of vitamin D deficiency. Measurement of 24,25(OH)2D3 is necessarily restricted to laboratories with mass spectrometry methods although cross reactivity of the metabolite in immunoassays for 25-OHD is a potential cause of misleading results. The international External Quality Assessment (EQA) scheme for vitamin D metabolites (DEQAS) was set up in 1989. In 2013 DEQAS became an accuracy based EQA for 25-OHD with 'target values' assigned by the National Institute of Standards and Technology (NIST) Reference Measurement Procedure (RMP). A pilot scheme for serum 24,25(OH)2D3 was started in 2015 and participants were asked to measure the metabolite on each of the 5 samples sent out for 25-OHD. Inter-laboratory agreement was poor but this may reflect methodological differences, in particular different approaches to assay standardization. An important potential contribution to reducing variability among assays was the development by NIST of a 24,25(OH)2D3 RMP and its use in assigning values to SRMs 972a, 2973 and 2971, supported by the NIH Office of Dietary Supplements (ODS) as part of the Vitamin D Standardization Program (VDSP) effort.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Carter, G. D.
Ahmed, F.
Berry, J.
Cavalier, Etienne ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Jongen, M.J.M., Ginkel, F.C.Van, Vijgh, W.J.Fvander, Kuiper, S., Netelenbos, J.C., Lips, P., An international comparison of Vitamin D metabolite measurements. Clin. Chem. 30 (1984), 399–403.
Mayer, E., Schmidt-Gayk, H., Inter-laboratory comparison of 25-hydroxyvitamin D determination. Clin. Chem. 30 (1984), 1199–1204.
Carter, G.D., Short, F., 25 - Hydroxyvitamin D assays: results of a national Quality Assessment. J. Endocrinol.(Suppl. 117), 1988 Abstract.
Burdette, C.Q., Camara, J.E., Sander, L.C., Carter, G.D., Jones, J., Betz, J.M., Sempos, C.T., Wise, S.A., Establishing an accuracy basis for the vitamin D external quality assessment scheme (DEQAS). J. AOAC Int. 100 (2017), 1277–1287.
Jones, G., Kaufmann, M., Vitamin D metabolite profiling using liquid chromatography–tandem mass spectrometry (LC–MS/MS). J. Steroid Biochem. Mol. Biol. 164 (2016), 110–114.
Cashman, K.D., Hayes, A., Galvin, K., Merkel, J., Jones, G., Kaufmann, M., Hoofnagle, A.N., Carter, G.D., Durazo-Arvizu, R.A., Sempos, C.T., Significance of serum 24,25-dihydroxyvitamin D in the assessment of vitamin D status: a double-edged sword?. Clin. Chem. 61 (2015), 636–645.
Carter, G.D., Jones, J.C., Berry, J.L., The anomalous behaviour of exogenous 25 hydroxyvitamin D in competitive binding assays. J. Steroid Biochem. Mol. Biol. 103 (2007), 480–482.
Horst, R., Exogenous versus endogenous recovery of 25-hydroxyvitamins D2 and D3 in human samples using high-performance liquid chromatography and the DiaSorin LIAISON Total-D Assay. J. Steroid Biochem. Mol. Biol. 121 (2010), 180–182.
National Committee for Clinical Laboratory Standards, Preparation and Validation of Commutable Frozen Human Serum Pools as Secondary Reference Materials for Cholesterol Measurement Procedures; Approved Guideline. NCCLS Document C-37A. 1999, NCCLS, Wayne, PA.
Carter, G.D., Jones, J.C., Shannon, J., Williams, E.L., Jones, G., Kaufmann, M., Sempos, C., 25-Hydroxyvitamin D assays: potential interference from other circulating vitamin D metabolites. J. Steroid Biochem. Mol. Biol. 164 (2016), 134–138.
Carter, G.D., Berry, J., Durazo-Arvizu, R., Gunter, E., Jones, G., Jones, J., Makin, H.L.J., Pattni, P., Sempos, C.T., Twomey, P., Williams, E.L., Wise, S.A., Hydroxyvitamin D assays: an historical perspective from DEQAS. J. Steroid Biochem. Mol. Biol. 177 (2018), 30–35.
Horst, R.L., Shepard, R.M., Jorgensen, N.A., DeLuca, H.F., The determination of 24,25-dihydroxyvitamin D and 25,26-dihydroxyvitamin D in plasma from normal and nephrectomiized man. J. Lab. Clin. Med. 93 (1979), 277–285.
Wise, S.A., Tai, S.S.-C., Nelson, M.A., Burdette, C.Q., Camara, J.E., Hoofnagle, A.N., Laha, T.J., Carter, G.D., Jones, J., Williams, E.L., Barclay, Z., Jones, G., Kaufmann, M., Binkley, N., Kapoor, A., Ziegler, T., Cashman, K.D., Dowling, K.G., Sempos, C.T., Interlaboratory comparison for the determination of 24,25-dihydroxyvitamin D 3 in human serum using liquid chromatography – tandem mass spectrometry. J. AOAC Int. 100 (2017), 1300–1317.
Tai, S.S.-C., Bedner, M., Phinney, K.W., Development of a candidate reference measurement procedure for the determination of 25 hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human serum using isotope-dilution. Anal. Chem. 82 (2010), 1942–1948.
Tai, S.S.-C., Nelson, M.A., Candidate reference measurement procedure for the determination of (24R), 25-dihydroxyvitamin D3 in human serum using isotope- dilution liquid chromatography−tandem mass spectrometry. Anal. Chem. 87 (2015), 7964–7970.
Kaufmann, M., Gallagher, J.C., Peacock, M., Schlingmann, K.-P., Konrad, M., DeLuca, H.F., Sigueiro, R., Lopez, B., Mourino, A., Maestro, A., St-Arnaud, R., Finkelstein, J.S., Cooper, D.P., Jones, G., Clinical utility of simultaneous quantitation of 25- hydroxyvitamin D and 24,25-dihydroxyvitamin D by LC–MS/MS involving derivatization with DMEQ-TAD. J. Clin. Endocrinol. Metab. 99 (2014), 2567–2574.
Kaufmann, M., Lee, S.M., Pike, J.W., Jones, G., A high-calcium, and phosphate rescue diet and VDR-expressing transgenes normalize serum vitamin D metabolite profiles and renal CYP27B1 and CYP24A1 expression in VDR-null mice. Endocrinology 156 (2015), 4388–4397.
Sempos, C.T., Heijboer, A.C., Bikle, D.D., Bollerslev, J., Bouillon, R., Brannon, P.M., DeLuca, H.F., Jones, G., Munns, C.F., Bilezikian, J.P., Giustina, A., Binkley, N., Vitamin D Assays and the Definition of Hypovitaminosis D: Results from the 1st International Conference on Controversies in Vitamin D. Br. J. Clin. Pharmacol. 84 (2018), 2194–2207.
www.deqas.org/document library/review 2017. [Accessed 8th August 2018].
Carter, G.D., Jones, J.C., Use of a common standard improves the performance of liquid chromatography-tandem mass spectrometry methods for serum 25-hydroxyvitamin-D. Ann. Clin. Biochem. 46 (2009), 79–81.
Tai, S.S.-C., Nelson, M.A., Bedner, M., Lang, B.E., Phinney, K.W., Sander, L.C., Yen, J.H., Betz, J.M., Sempos, C.T., Wise, S.A., Development of standard reference material (SRM) 2973 vitamin D metabolites in frozen human serum (high level). J. AOAC Int. 100 (2017), 1294–1301.
Certificate of Analysis. Standard reference material 2971. 24R,25-Dihydroxyvitamin D 3 Calibrating Solution. Available at https://www-s.nist.gov/srmors/view_cert.cfm?srm=2971 [Accessed 21 August 2018].
http://www.ifcc.org/ifcc-scientific-division/ Vitamin D Standardization Program (WG-Vit D) (A ccessed 20 August 2018).