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Article (Scientific journals)
Comparison of the effects of ethidium bromide and of ethidium bromide-deoxyribonucleic acid complex in fibroblasts cultivated in vitro.
Heinen, Ernst; Bassleer, Roger; Calberg-Bacq, Claire-Michelle
1976In Beitrage zur Pathologie, 159 (2), p. 207-18
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Keywords :
Animals; Antineoplastic Agents/administration & dosage; Cells, Cultured; Chick Embryo; DNA/therapeutic use; Ethidium/administration & dosage/metabolism; Fibroblasts/drug effects/metabolism; Lysosomes; Mitosis/drug effects
Abstract :
[en] Chick embryo fibroblasts cultivated in vitro were treated with ethidium bromide (E.B.) or with DNA-E.B. complex (DNA-E.B.). E.B. (5 mug/ml) provokes morphological alterations and cell death, inhibits DNA synthesis and mitotic activity. DNA-E.B. (E.B. 5 mug/ml) is less toxic to the fibroblasts as far as cell structure, DNA synthesis and mitotic activity are concerned. DNA alone has no apparent effect on the fibroblasts. As shown by fluorescence microscopy, the lower toxicity of DNA-E.B. seems to be related to its mode of penetration into the cells.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Bassleer, Roger ;  Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Calberg-Bacq, Claire-Michelle;  Université de Liège - ULiège
Language :
English
Title :
Comparison of the effects of ethidium bromide and of ethidium bromide-deoxyribonucleic acid complex in fibroblasts cultivated in vitro.
Publication date :
1976
Journal title :
Beitrage zur Pathologie
ISSN :
0005-8165
Publisher :
Fischer, Stuttgart, Germany
Volume :
159
Issue :
2
Pages :
207-18
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 September 2009

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