Reference : Comparison of the effects of ethidium bromide and of ethidium bromide-deoxyribonuclei...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Comparison of the effects of ethidium bromide and of ethidium bromide-deoxyribonucleic acid complex in fibroblasts cultivated in vitro.
Heinen, Ernst mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine >]
Bassleer, R. [> > > >]
Calberg, C.-M. [ > > ]
Beitrage zur Pathologie
Yes (verified by ORBi)
[en] Animals ; Antineoplastic Agents/administration & dosage ; Cells, Cultured ; Chick Embryo ; DNA/therapeutic use ; Ethidium/administration & dosage/metabolism ; Fibroblasts/drug effects/metabolism ; Lysosomes ; Mitosis/drug effects
[en] Chick embryo fibroblasts cultivated in vitro were treated with ethidium bromide (E.B.) or with DNA-E.B. complex (DNA-E.B.). E.B. (5 mug/ml) provokes morphological alterations and cell death, inhibits DNA synthesis and mitotic activity. DNA-E.B. (E.B. 5 mug/ml) is less toxic to the fibroblasts as far as cell structure, DNA synthesis and mitotic activity are concerned. DNA alone has no apparent effect on the fibroblasts. As shown by fluorescence microscopy, the lower toxicity of DNA-E.B. seems to be related to its mode of penetration into the cells.

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