Reference : Paclitaxel-loaded multifunctional nanoparticles for the targeted treatment of glioblastoma
Scientific journals : Article
Engineering, computing & technology : Materials science & engineering
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/231810
Paclitaxel-loaded multifunctional nanoparticles for the targeted treatment of glioblastoma
English
Ganipineni, Lakshmi Pallavi [Université catholique de Louvain (UCL), Brussels, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials > > > >]
Ucakar, Bernard [Université catholique de Louvain (UCL), Brussels, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials > > > > >]
Joudiou, Nicolas [Université catholique de Louvain (UCL), Brussels, Louvain Drug Research Institute, Nuclear and Electron Spin Technologies Platform (NEST) > > > >]
Riva, Raphaël mailto [University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > > > >]
Jérôme, Christine mailto [University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > > > >]
Gallez, Bernard [Université catholique de Louvain (UCL), Brussels, Louvain Drug Research Institute, Nuclear and Electron Spin Technologies Platform (NEST) > > > >]
Danhier, Fabienne [Université catholique de Louvain (UCL), Brussels, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials > > > >]
Préat, Véronique [Université catholique de Louvain (UCL), Brussels, Louvain Drug Research Institute, Advanced Drug Delivery and Biomaterials > > > >]
2019
Journal of Drug Targeting
Taylor & Francis
Yes (verified by ORBi)
International
1061-186X
1029-2330
United Kingdom
[en] biomaterial ; nanomedicine
[en] We hypothesized that the active targeting of αvβ3 integrin overexpressed in neoangiogenic blood vessels and glioblastoma (GBM) cells combined with magnetic targeting of paclitaxel- and SPIO-loaded PLGA-based nanoparticles could improve accumulation of nanoparticles in the tumor and therefore improve the treatment of GBM. Methods: PTX/SPIO PLGA nanoparticles with or without RGD-grafting were characterized. Their in vitro cellular uptake and cytotoxicity was evaluated by fluorospectroscopy and MTT assay. In vivo safety and anti-tumor efficacy of different targeting strategies was evaluated in orthotopic U87MG tumor model over multiple intravenous injections. Results: The nanoparticles of 250nm were negatively charged. RGD targeted nanoparticles showed a specific and higher cellular uptake than untargeted nanoparticles by activated U87MG and HUVEC cells. In vitro IC50 of PTX after 48h was approximately 1 ng/mL for all the PTX-loaded nanoparticles. The median survival time of the mice treated with magnetic targeted nanoparticles was higher than the control (saline) mice or mice treated with other evaluated strategies. The 6 doses of PTX did not induce any detectable toxic effects on liver, kidney and heart when compared to Taxol. Conclusion. The magnetic targeting strategy resulted in better therapeutic effect than the other targeting strategies (passive, active).
Complex and Entangled Systems from Atoms to Materials (CESAM) ; Center for Education and Research on Macromolecules (CERM)
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Télévie ; Fondation contre le Cancer
Researchers
http://hdl.handle.net/2268/231810
10.1080/1061186X.2019.1567738

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