Subunit Asa3 ensures the attachment of the peripheral stalk to the membrane sector of the dimeric ATP synthase of Polytomella sp.

Colina-Tenorio, Lilia; Miranda Astudillo, Héctor Vicente; Dautant, Alain; Vazquez-Acevedo, Miriam; Giraud, Marie-France; Gonzalez-Halphen, Diego

doi:10.1016/j.bbrc.2018.12.142

Article (Scientific journals)

Subunit Asa3 ensures the attachment of the peripheral stalk to the membrane sector of the dimeric ATP synthase of Polytomella sp.

Colina-Tenorio, Lilia; Miranda Astudillo, Héctor Vicente; Dautant, Alain et al.

2019 • In Biochemical and Biophysical Research Communications, 509 (2), p. 341-347

Peer reviewed

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https://hdl.handle.net/2268/231554

DOI
10.1016/j.bbrc.2018.12.142

PubMed
30585150

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Keywords :

ATP synthase; Asa subunit; HEAT repeats; Peripheral stalk; Polytomella

Abstract :

[en] The mitochondrial ATP synthase of Polytomella exhibits a peripheral stalk and a dimerization domain built by the Asa subunits, unique to chlorophycean algae. The topology of these subunits has been extensively studied. Here we explored the interactions of subunit Asa3 using Far Western blotting and subcomplex reconstitution, and found it associates with Asa1 and Asa8. We also identified the novel interactions Asa1-Asa2 and Asa1-Asa7. In silico analyses of Asa3 revealed that it adopts a HEAT repeat- like structure that points to its location within the enzyme based on the available 3D-map of the algal ATP synthase. We suggest that subunit Asa3 is instrumental in securing the attachment of the peripheral stalk to the membrane sector, thus stabilizing the dimeric mitochondrial ATP synthase.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Colina-Tenorio, Lilia; Universidad Nacional Autonoma de Mexico > Instituto de Fisiología Celular > Departamento de Genetica Molecular

Miranda Astudillo, Héctor Vicente ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues

Dautant, Alain; Universite de Bordeaux > Institut de Biochimie et Genetique Cellulaires > Energy Transducing Systems and Mitochondrial Morphology

Vazquez-Acevedo, Miriam; Universidad Nacional Autonoma de Mexico > Instituto de Fisiología Celular > Departamento de Genetica Molecular

Giraud, Marie-France; Universite de Bordeaux > Institut de Biochimie et Genetique Cellulaires > Energy Transducing Systems and Mitochondrial Morphology

Gonzalez-Halphen, Diego; Universidad Nacional Autonoma de Mexico > Instituto de Fisiología Celular > Departamento de Genetica Molecular

Language :

English

Title :

Subunit Asa3 ensures the attachment of the peripheral stalk to the membrane sector of the dimeric ATP synthase of Polytomella sp.

Publication date :

05 February 2019

Journal title :

Biochemical and Biophysical Research Communications

ISSN :

0006-291X

eISSN :

1090-2104

Publisher :

Elsevier, Atlanta, United States - California

Volume :

509

Issue :

Pages :

341-347

Peer reviewed :

Peer reviewed

Available on ORBi :

since 16 January 2019

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