magnetic polyion complex micelle; cytotoxicity; cell internalization
Abstract :
[en] Magnetic nanoparticles (MNPs) of magnetite (Fe3O4) were prepared using a polystyrene-graft-poly(2-vinylpyridine) copolymer (denoted G0PS-g-P2VP or G1) as template. These MNPs were subjected to self-assembly with a poly(acrylic acid)-block-poly(2-hydroxyethyl acrylate) double-hydrophilic block copolymer (DHBC), PAA-b-PHEA, to form water-dispersible magnetic polyion complex (MPIC) micelles. Large Fe3O4 crystallites were visualized by transmission electron microscopy (TEM) and magnetic suspensions of MPIC micelles exhibited improved colloidal stability in aqueous environments over a wide pH and ionic strength range. L929 and U87 cell lines incubated for 48 h with MPIC micelles at the highest concentration (1250 µg of Fe3O4 per mL) had a cell viability of 91%, as compared with 51% when incubated with bare (unprotected) MNPs. Cell internalization, visualized by confocal laser scanning microscopy (CLSM) and TEM, exhibited strong dependence on the MPIC micelle concentration and incubation time, as also evidenced by fluorescence-activated cell sorting (FACS). The usefulness of MPIC micelles for cellular radiofrequency magnetic field hyperthermia (MFH) was also confirmed, as the MPIC micelles showed a dual dose-dependent effect (concentration and duration of magnetic field exposure) on the viability of L929 mouse fibroblasts and U87 human glioblastoma epithelial cells.
Research center :
MolSys - Molecular Systems - ULiège
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Nguyen, Vo Thu An; University Bordeaux > UMR 5629 > Laboratoire de Chimie des Polymères Organiques
Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
Gauthier, Mario; University of Waterloo > Department of Chemistry
Sandre, Olivier; University Bordeaux > CNRS > Laboratoire de Chimie des Polymères Organiques
Language :
English
Title :
Magnetic Polyion Complex Micelles for Cell Toxicity Induced by Radiofrequency Magnetic Field Hyperthermia
Publication date :
06 December 2018
Journal title :
Nanomaterials
eISSN :
2079-4991
Publisher :
MDPI AG, Basel, Switzerland
Special issue title :
Magnetic Nanoparticles in Biological Applications
Volume :
8
Issue :
12
Pages :
1014
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Multifunctional Nanoparticles for Magnetic Hyperthermia and Indirect Radiation Therapy
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