Reference : Determination of dimethylsulfoniopropionate and dimethylsulfoxide in Posidonia oceani...
Scientific journals : Article
Life sciences : Aquatic sciences & oceanology
http://hdl.handle.net/2268/230651
Determination of dimethylsulfoniopropionate and dimethylsulfoxide in Posidonia oceanica leaf tissue
English
Champenois, Willy mailto [Université de Liège - ULiège > Département d'astrophys., géophysique et océanographie (AGO) > Chemical Oceanography Unit (AGO) >]
Borges, Alberto mailto [Université de Liège - ULiège > Département d'astrophys., géophysique et océanographie (AGO) > Chemical Oceanography Unit (AGO) >]
1-Jan-2019
MethodsX
Elsevier
6
56–62
Yes (verified by ORBi)
International
2215-0161
Netherlands
[en] In order to investigate the possible use of the dimethylsulfoniopropionate (DMSP) and dimethylsulfoxide (DMSO)
ratio as a stress indicator of Posidonia oceanica a method for the determination of these quantities was developed
for this type of material.
The method relies on gas chromatography with headspace technique, instead of the purge-and-trap technique
commonly used.
The method allows the determination of both DMSP and DMSO on the same sample.
This method allows to quantify DMSP, DMSO and DMSP:DMSO ratio for calibration curves with a coefficient of
variation around 2% and a relative error around 2% and within the ranges natural variability of DMSP and DMSO
in P. oceanica leaf tissue.
Preliminary tests showed that DMSP in P. oceanica leaf tissue ranged from 20 to 200 mmol g 1 of fresh weight
(FW) and 2 to 5 mmol gfw
1 for DMSO. The DMSP:DMSO ratio ranged from 2 to 40. The quantifications were
conducted with different mixtures of DMSP and DMSO by measurements of DMSP and DMSO in the same sample
of P. oceanica leaf tissue.
Freshwater and OCeanic science Unit of reSearch - FOCUS
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/230651
10.1016/j.mex.2018.12.014

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