Reference : Short communication: Effect of freezer storage time and thawing method on the recover...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
Short communication: Effect of freezer storage time and thawing method on the recovery of Mycoplasma bovis from bovine colostrum.
Gille, Linde mailto [Université de Liège - ULiège > > CVU : pôle ruminants-porcs >]
Boyen, F. [> >]
Van Driessche, L. [> >]
Valgaeren, B. [> >]
Haesebrouck, F. [> >]
Deprez, P. [> >]
Pardon, B. [> >]
Journal of Dairy Science
Yes (verified by ORBi)
United States
[en] Animals ; Cattle ; Colostrum/microbiology ; Female ; Freezing ; Longitudinal Studies ; Mastitis, Bovine/microbiology ; Mycoplasma bovis/isolation & purification/physiology ; Pregnancy ; Mycoplasma bovis ; colostrum ; freezing ; survival
[en] Mycoplasma bovis is an important cause of mastitis in dairy cattle, and pneumonia, arthritis, and otitis in calves. Milk and colostrum are considered important sources of infection for calves. knowledge on the effect of on-farm freezing (-18 degrees C) and thawing methods on the recovery of M. bovis from colostrum samples is missing. In this study, 2 separate experiments were performed. The first experiment consisted of a longitudinal study examining the survival [as measured by log(10) reduction] of 2 M. bovis strains in frozen colostrum over 14 wk. The second experiment examined the effect of different thawing temperatures (45 and 20 degrees C), thawing frequencies (once or twice), and initial colostrum titer (10(4) or 10(6) cfu/mL) on M. bovis survival. A single freeze-thaw cycle led to an approximate 1 log reduction of M. bovis titer, independent of the thawing temperature. Freezing for 14 wk did not significantly further reduce the titer of bacteria compared with freezing for 2 wk. A second freeze-thaw cycle further reduced the M. bovis count by approximately 0.5 log compared with a single freeze-thaw cycle. Thawing temperature and initial bacterial concentration did not significantly affect M. bovis reduction. In conclusion, storage of colostrum samples in the freezer at -18 degrees C during epidemiological studies, herd monitoring, or test and cull programs will probably have little influence on qualitative bacteriological test results for M. bovis. The epidemiological or clinical relevance of an approximate 1 log reduction of M. bovis in colostrum is currently unclear.
Copyright (c) 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

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