Article (Scientific journals)
Short communication: Effect of freezer storage time and thawing method on the recovery of Mycoplasma bovis from bovine colostrum.
Gille, Linde; Boyen, F.; Van Driessche, L. et al.
2018In Journal of Dairy Science, 101 (1), p. 609-613
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Keywords :
Animals; Cattle; Colostrum/microbiology; Female; Freezing; Longitudinal Studies; Mastitis, Bovine/microbiology; Mycoplasma bovis/isolation & purification/physiology; Pregnancy; Mycoplasma bovis; colostrum; survival
Abstract :
[en] Mycoplasma bovis is an important cause of mastitis in dairy cattle, and pneumonia, arthritis, and otitis in calves. Milk and colostrum are considered important sources of infection for calves. knowledge on the effect of on-farm freezing (-18 degrees C) and thawing methods on the recovery of M. bovis from colostrum samples is missing. In this study, 2 separate experiments were performed. The first experiment consisted of a longitudinal study examining the survival [as measured by log(10) reduction] of 2 M. bovis strains in frozen colostrum over 14 wk. The second experiment examined the effect of different thawing temperatures (45 and 20 degrees C), thawing frequencies (once or twice), and initial colostrum titer (10(4) or 10(6) cfu/mL) on M. bovis survival. A single freeze-thaw cycle led to an approximate 1 log reduction of M. bovis titer, independent of the thawing temperature. Freezing for 14 wk did not significantly further reduce the titer of bacteria compared with freezing for 2 wk. A second freeze-thaw cycle further reduced the M. bovis count by approximately 0.5 log compared with a single freeze-thaw cycle. Thawing temperature and initial bacterial concentration did not significantly affect M. bovis reduction. In conclusion, storage of colostrum samples in the freezer at -18 degrees C during epidemiological studies, herd monitoring, or test and cull programs will probably have little influence on qualitative bacteriological test results for M. bovis. The epidemiological or clinical relevance of an approximate 1 log reduction of M. bovis in colostrum is currently unclear.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Gille, Linde  ;  Université de Liège - ULiège > CVU : pôle ruminants-porcs
Boyen, F.
Van Driessche, L.
Valgaeren, B.
Haesebrouck, F.
Deprez, P.
Pardon, B.
Language :
English
Title :
Short communication: Effect of freezer storage time and thawing method on the recovery of Mycoplasma bovis from bovine colostrum.
Publication date :
2018
Journal title :
Journal of Dairy Science
ISSN :
0022-0302
eISSN :
1525-3198
Publisher :
American Dairy Science Association, United States - Illinois
Volume :
101
Issue :
1
Pages :
609-613
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
Available on ORBi :
since 21 December 2018

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