Poster (Scientific congresses and symposiums)
Characterization of putative acetate transporters in Chlamydomonas reinhardtii
Durante, Lorenzo; Hanikenne, Marc; Remacle, Claire
2016XX school of pure and applied biophysics
 

Files


Full Text
Durante L_poster_Venezia_jan 2016CR_(new).pdf
Author postprint (270.43 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Chlamydomonas reinhardtii; Acetate metabolism; amiRNA
Abstract :
[en] The unicellular green alga C. reinhardtii can grown either phototrophically with CO2 as the sole carbon source, heterotrophically by consuming acetate in the dark and mixotrophically by using both carbon sources in the light. Despite significant knowledge gained on acetate metabolism, the genes coding for acetate transporter/permease are still unknown in this alga. However, recent analyses1,2 have shown five functionally uncharacterized members of the GPR1/FUN34/yaaH (GFY), a protein family which includes genes involved in carboxylic organic acid uptake/sensing already described in bacteria, yeasts and filamentous fungi. Thus, the five genes identified in C. reinhardtii as Cre17.g700450 (GFY1), Cre17.g.700650 (GFY2), Cre17.g.700750 (GFY3), Cre17.g.702900 (GFY4) and Cre17.g.702950 (GFY5) encode for putative acetate transporter proteins given that they are structured in 6 hydrophobic transmembrane helices. They are characterized by a close gene structure (Fig. 1) and very high similarity in their coding sequence (CDS) except for a clear distinction at the N-terminus amino acid sequences (Fig. 2). A reverse functional genomics approach by using artificial micro RNA (amiRNA) gene silencing was adopted to target the five genes one-by-one. Until now, ~160 transformants were generated for each amiRNA construct and their characterization is ongoing. A further characterization of the mutants will follow to have an understanding of the gene function in the acetate metabolism.
Research center :
Génétique et Physiologie des Microalgues
Disciplines :
Biotechnology
Author, co-author :
Durante, Lorenzo ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Hanikenne, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Remacle, Claire  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues
Language :
English
Title :
Characterization of putative acetate transporters in Chlamydomonas reinhardtii
Publication date :
January 2016
Event name :
XX school of pure and applied biophysics
Event organizer :
SIBPA - scuola italiana biofisica pura e applicata
Event date :
from 25/1/2016 to 29/1/2016
Audience :
International
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 21 December 2018

Statistics


Number of views
119 (5 by ULiège)
Number of downloads
47 (8 by ULiège)

Bibliography


Similar publications



Contact ORBi