Reference : Characterization of putative acetate transporters in Chlamydomonas reinhardtii
Scientific congresses and symposiums : Poster
Life sciences : Biotechnology
http://hdl.handle.net/2268/230379
Characterization of putative acetate transporters in Chlamydomonas reinhardtii
English
Durante, Lorenzo mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues >]
Hanikenne, Marc mailto [Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale >]
Remacle, Claire mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues >]
Jan-2016
No
International
XX school of pure and applied biophysics
from 25/1/2016 to 29/1/2016
SIBPA - scuola italiana biofisica pura e applicata
[en] Chlamydomonas reinhardtii ; Acetate metabolism ; amiRNA
[en] The unicellular green alga C. reinhardtii can grown either phototrophically with CO2 as the sole carbon source, heterotrophically by consuming acetate in the dark and mixotrophically by using both carbon sources in the light. Despite significant knowledge gained on acetate metabolism, the genes coding for acetate transporter/permease are still unknown in this alga. However, recent analyses1,2 have shown five functionally uncharacterized members of the GPR1/FUN34/yaaH (GFY), a protein family which includes genes involved in carboxylic organic acid uptake/sensing already described in bacteria, yeasts and filamentous fungi. Thus, the five genes identified in C. reinhardtii as Cre17.g700450 (GFY1), Cre17.g.700650 (GFY2), Cre17.g.700750 (GFY3), Cre17.g.702900 (GFY4) and Cre17.g.702950 (GFY5) encode for putative acetate transporter proteins given that they are structured in 6 hydrophobic transmembrane helices. They are characterized by a close gene structure (Fig. 1) and very high similarity in their coding sequence (CDS) except for a clear distinction at the N-terminus amino acid sequences (Fig. 2).
A reverse functional genomics approach by using artificial micro RNA (amiRNA) gene silencing was adopted to target the five genes one-by-one. Until now, ~160 transformants were generated for each amiRNA construct and their characterization is ongoing. A further characterization of the mutants will follow to have an understanding of the gene function in the acetate metabolism.
Génétique et Physiologie des Microalgues
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
http://hdl.handle.net/2268/230379

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