Article (Scientific journals)
Linoleic and linolenic acid hydroperoxides interact differentially with biomimetic plant membranes in a lipid specific manner
Deleu, Magali; Deboever, Estelle; Nasir, Mehmet Nail et al.
2019In Colloids and Surfaces B: Biointerfaces, 175, p. 384-391
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Keywords :
Oxylipins; plant membrane; molecular interactions; lipid specificity
Abstract :
[en] Linoleic and linolenic acid hydroperoxides (HPOs) constitute key intermediate oxylipins playing an important role as signaling molecules during plant defense processes in response to biotic or abiotic stress. They have also been demonstrated in vitro as antimicrobial agents against plant fungi and bacteria. To reach the phytopathogens in vivo, the HPOs biosynthesized in the plant cells must cross the plant plasma membrane (PPM) where they can also interact with plasma membrane lipids and have an effect on their organization.In the present study, we have investigated the interaction properties of HPOs with PPM at a molecular level using biophysical tools combining in vitro and in silico approaches and using plant biomimetic lipid systems. Our results have shown that HPOs are able to interact with PPM lipids and perturb their lateral organization. Glucosylceramide (GluCer) is a privileged partner, sitosterol lessens their binding and the presence of both GluCer and sitosterol further reduces their interaction. Hydrophobic effect and polar interactions are involved in the binding. The chemical structure of HPOs influences their affinity for PPM lipids. The presence of three double bonds in the HPO molecule gives rise to a higher affinity comparatively to two double bonds, which can be explained by their differential interaction with the lipid polar headgroups.
Disciplines :
Chemistry
Agriculture & agronomy
Biochemistry, biophysics & molecular biology
Author, co-author :
Deleu, Magali   ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Deboever, Estelle  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Nasir, Mehmet Nail 
Crowet, Jean-Marc ;  Université de Reims Champagne-Ardenne
Dauchez, Manu;  Université de Reims Champagne-Ardenne,
Ongena, Marc ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Jijakli, Haissam  ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Gestion durable des bio-agresseurs
Fauconnier, Marie-Laure   ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
Lins, Laurence   ;  Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes
 These authors have contributed equally to this work.
Language :
English
Title :
Linoleic and linolenic acid hydroperoxides interact differentially with biomimetic plant membranes in a lipid specific manner
Publication date :
2019
Journal title :
Colloids and Surfaces B: Biointerfaces
ISSN :
0927-7765
Publisher :
Elsevier, Netherlands
Volume :
175
Pages :
384-391
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
ARC-FIELD project 13/17-10
Funders :
ULg. ARC - Université de Liège. Actions de Recherche Concertées [BE]
Available on ORBi :
since 13 December 2018

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