Collision induced unfolding; CIU; Collision induced dissociation; CID; Electron transfer dissociation; ETD; Synthetic polymers; Ion mobility calibration
Abstract :
[en] Polymer characterizations are often performed using mass spectrometry (MS). Aside from MS and different tandem MS (MS/MS) tech- niques, ion mobility–mass spectrometry (IM-MS) has been recently added to the inventory of char- acterization technique. However, only few studies have focused on the reproducibility and robust- ness of polymer IM-MS analyses. Here, we per- form collisional and electron-mediated activation of polymer ions before measuring IM drift times, collision cross-sections (CCS), or reduced ion mobilities (K0). The resulting IM behavior of different activated product ions is then compared to non-activated native intact polymer ions. First, we analyzed collision induced unfolding (CIU) of precursor ions to test the robustness of polymer ion shapes. Then, we focused on fragmen- tation product ions to test for shape retentions from the precursor ions: cation ejection species (CES) and product ions with m/z and charge state values identical to native intact polymer ions. The CES species are formed using both collision induced dissociation (CID) and electron transfer dissociation (ETD, formally ETnoD) experiments. Only small drift time, CCS, or K0 deviations between the activated/formed ions are observed compared to the native intact polymer ions. The polymer ion shapes seem to depend solely on their mass and charge state. The experiments were performed on three synthetic homopolymers: poly(ethoxy phosphate) (PEtP), poly(2-n-propyl- 2-oxazoline) (Pn-PrOx), and poly(ethylene oxide) (PEO). These results confirm the robustness of polymer ion CCSs for IM calibration, especially singly charged polymer ions. The results are also discussed in the context of polymer analyses, CCS predictions, and probing ion–drift gas interaction potentials.
Research center :
MolSys - Molecular Systems - ULiège CESAM - Complex and Entangled Systems from Atoms to Materials - ULiège Supramolecular Chemistry Group
Disciplines :
Chemistry
Author, co-author :
Haler, Jean ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Massonnet, Philippe ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Far, Johann ; Université de Liège - ULiège > Département de chimie (sciences) > Center for Analytical Research and Technology (CART)
de la Rosa, Victor; Ghent University > Supramolecular Chemistry Group
Lecomte, Philippe ; Université de Liège - ULiège > Département de chimie (sciences) > Centre d'études et de rech. sur les macromolécules (CERM)
Hoogenboom, Richard; Ghent University > Supramolecular Chemistry Group
Jérôme, Christine ; Université de Liège - ULiège > Département de chimie (sciences) > Centre d'études et de rech. sur les macromolécules (CERM)
De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Language :
English
Title :
Gas-Phase Dynamics of Collision Induced Unfolding, Collision Induced Dissociation, and Electron Transfer Dissociation-Activated Polymer Ions
Publication date :
April 2019
Journal title :
Journal of the American Society for Mass Spectrometry
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE] FWO - Fonds Wetenschappelijk Onderzoek Vlaanderen [BE] UGent - Universiteit Gent [BE]
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