Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels

[en] Genetic engineering is considered to be an important tool for the improvement of cassava. Cassava is a highly heterozygous crop species for which conventional breeding is a lengthy and tedious process. Robust transformation is based on Agrobacterium-mediated transformation of friable embryogenic callus (FEC). Production of FEC is genotype-dependent and considered to be a major bottleneck for the genetic transformation of cassava. As a consequence, routine genetic transformation has only been established for a handful of cassava cultivars. Therefore, development of procedures enabling efficient production of high-quality cassava FEC is required to allow the translation of research from the model cultivar to farmer-preferred cassava cultivars. Here we study the FEC production capacity of Brazilian cassava cultivars and report the modification of the protocol for the genetic transformation of Verdinha (BRS 222), a recalcitrant cultivar with high potential for protein production that is extensively used by farmers in Brazil.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Lentz, Ezequiel

Eisner, Sabrina

McCallum, Emily Jane

Schlegel, Kim

de Assis de Paiva Campos, Francisco

Gruissem, Wilhelm

Vanderschuren, Hervé ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions végétales et valorisation

Language :

English

Title :

Genetic Transformation of Recalcitrant Cassava by Embryo Selection and Increased Hormone Levels

Publication date :

2018

Journal title :

Methods and Protocols

eISSN :

2409-9279

Publisher :

MDPI AG, Switzerland

Volume :

1, 42

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 November 2018

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