Wireless multichannel optogenetic stimulators enabled by narrow bandwidth resonant tank circuits

Channelrhodopsin; Human embryonic kidney cells; In-vitro; Inductive; Multichannel; Optogenetics; Retinal ganglion cells; Wireless power; Cells; Chemical activation; Cytology; Diseases; Implants (surgical); Inductive power transmission; Light sources; Neurons; Ophthalmology; Power transmission; Timing circuits; Resonant circuits

Abstract :

[en] Optogenetic neuromodulation is a powerful technique used to study cells that form part of neuronal circuits. Light stimulation of neurons has led to a deeper understanding of autism, schizophrenia and depression. However, researchers are often limited to tethered systems involving percutaneous plugs, hence, wireless power transmission to an implantable device is desirable. This work details the design, fabrication and testing of multichannel wirelessly powered optogenetic devices. By employing several carefully tuned resonant tank circuits, this work demonstrates the ability to address a scalable number of light sources on a single device. Single channel, dual channel and 16 channel devices were fabricated, achieving light output readings of up to 15mW at 473nm, suitable for activating channelrhodopsin. Wireless power transmission was characterized in air and porcine tissue for implant depths up to 30mm, making device implantation feasible. The device was successful in activating endogenous (in retinal ganglion cells) and exogenously transfected channelrhodopsin in human embryonic kidney cells, providing biological validation. The significance of this approach is the removal of power-hungry and area-consuming electronics from the implant, while the ability to address and modulate individual light sources is maintained by shifting this complexity to the external wireless power transmitter. © 2017 Elsevier B.V.

Disciplines :

Electrical & electronics engineering

Author, co-author :

Aldaoud, A.; School of Physics, University of Melbourne, Parkville, Victoria, Australia

Soto-Breceda, A.; National Vision Research Institute, Australian College of Optometry, Carlton, Victoria, Australia, Department of Biomedical Engineering, University of Melbourne, Parkville, Victoria, Australia, Data 61, CSIRO, Docklands, Victoria, Australia

Tong, Wenyi ; Université de Liège - ULiège > Département de physique > Physique théorique des matériaux

Conductier, G.; Department of Physiology and Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

Tonta, M. A.; Department of Physiology and Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

Coleman, H. A.; Department of Physiology and Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

Parkington, H. C.; Department of Physiology and Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

Clarke, I.; Department of Physiology and Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia

Redouté, Jean-Michel ; Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes microélectroniques intégrés

Garrett, D. J.; School of Physics, University of Melbourne, Parkville, Victoria, Australia

Prawer, S.; School of Physics, University of Melbourne, Parkville, Victoria, Australia

Language :

English

Title :

Wireless multichannel optogenetic stimulators enabled by narrow bandwidth resonant tank circuits

Publication date :

2018

Journal title :

Sensors and Actuators. A, Physical

ISSN :

0924-4247

eISSN :

1873-3069

Publisher :

Elsevier B.V.

Volume :

271

Pages :

201-211

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

GNT1101717, NHMRC, National Health and Medical Research Council

Available on ORBi :

since 15 November 2018

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