Polycytidylation of mitochondrial mRNAs in Chlamydomonas reinhardtii

Chlamydomonas reinhardtii; Base Sequence; Chlorophyta; Genome, Mitochondrial; Mitochondria; Phylogeny; Poly C; RNA, Messenger; Sequence Homology, Nucleic Acid; Transcription, Genetic

Abstract :

[en] The unicellular photosynthetic organism, Chlamydomonas reinhardtii, represents a powerful model to study mitochondrial gene expression. Here, we show that the 5'- and 3'-extremities of the eight Chlamydomonas mitochondrial mRNAs present two unusual characteristics. First, all mRNAs start primarily at the AUG initiation codon of the coding sequence which is often marked by a cluster of small RNAs. Second, unusual tails are added post-transcriptionally at the 3'-extremity of all mRNAs. The nucleotide composition of the tails is distinct from that described in any other systems and can be partitioned between A/U-rich tails, predominantly composed of Adenosine and Uridine, and C-rich tails composed mostly of Cytidine. Based on 3' RACE experiments, 22% of mRNAs present C-rich tails, some of them composed of up to 20 consecutive Cs. Polycytidylation is specific to mitochondria and occurs primarily on mRNAs. This unprecedented post-transcriptional modification seems to be a specific feature of the Chlorophyceae class of green algae and points out the existence of novel strategies in mitochondrial gene expression. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Salinas-Giegé, T.; Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67084 Strasbourg, France

Cavaiuolo, M.; UMR 7141, CNRS, Université Pierre et Marie Curie, Institut de Biologie Physico-Chimique, 75005 Paris, France

Cognat, V.; Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67084 Strasbourg, France

Ubrig, E.; Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67084 Strasbourg, France

Remacle, Claire ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues

Duchêne, A.-M.; Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67084 Strasbourg, France

Vallon, O.; UMR 7141, CNRS, Université Pierre et Marie Curie, Institut de Biologie Physico-Chimique, 75005 Paris, France

Maréchal-Drouard, L.; Institut de biologie moléculaire des plantes, CNRS, Université de Strasbourg, 67084 Strasbourg, France

Language :

English

Title :

Polycytidylation of mitochondrial mRNAs in Chlamydomonas reinhardtii

Publication date :

2017

Journal title :

Nucleic Acids Research

ISSN :

0305-1048

eISSN :

1362-4962

Publisher :

Oxford University Press, Oxford, United Kingdom

Volume :

Issue :

Pages :

12963-12973

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 08 November 2018

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