Reference : In situ photochemical crosslinking of hydrogel membrane for guided tissue regeneration
Scientific journals : Article
Engineering, computing & technology : Materials science & engineering
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/228644
In situ photochemical crosslinking of hydrogel membrane for guided tissue regeneration
English
Chichiricco, Pauline Marie [University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > University of Nantes, Inserm, Regenerative Medicine and Skeleton, France > > >]
Riva, Raphaël [University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > > > >]
Thomassin, Jean-Michel [Université de Liège - ULiège > Département de chimie (sciences) > Centre d'études et de rech. sur les macromolécules (CERM) >]
Lesoeur, Julie [University of Nantes, Inserm, Regenerative Medicine and Skeleton, UFR d'Odontologie, France > CHU Nantes, Structure Fédérative de Recherche François Bonamy > > > >]
Struillou, Xavier [University of Nantes, Inserm, Regenerative Medicine and Skeleton, UFR d'Odontologie, France > CHU Nantes, OTONN, France > > >]
Le Visage, Catherine [University of Nantes, Inserm, Regenerative Medicine and Skeleton, UFR d'Odontologie, France > > > >]
Jérôme, Christine mailto [University of Liège (ULiège), Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM) > > > >]
Weiss, Pierre [University of Nantes, Inserm, Regenerative Medicine and Skeleton, UFR d'Odontologie, France > CHU Nantes, OTONN, France > > >]
2018
Dental Materials
Elsevier
Yes (verified by ORBi)
International
0109-5641
1879-0097
Netherlands
[en] biomaterial ; scaffold
[en] Periodontitis is an inflammatory disease that destroys the tooth-supporting
attachment apparatus. Guided tissue regeneration (GTR) is a technique based on a bar- rier membrane designed to prevent wound space colonization by gingival cells. This study examined a new formulation composed of two polymers that could be photochemically cross-linked in situ into an interpenetrated polymer network (IPN) forming a hydrogel mem- brane. Methods. We synthetized and characterized silanized hydroxypropyl methylcellulose (Si- HPMC) for its cell barrier properties and methacrylated carboxymethyl chitosan (MA-CMCS) for its degradable backbone to use in IPN. Hydrogel membranes were cross-linked using riboflavin photoinitiator and a dentistry visible light lamp. The biomaterial’s physicochem- ical and mechanical properties were determined. Hydrogel membrane degradation was evaluated in lysozyme. Cytocompatibility was estimated by neutral red uptake. The cell bar- rier property was studied culturing human primary gingival fibroblasts or human gingival explants on membrane and analyzed with confocal microscopy and histological staining. Results. The IPN hydrogel membrane was obtained after 120 s of irradiation. The IPN showed a synergistic increase in Young moduli compared with the single networks. The CMCS addition in IPN allows a progressive weight loss compared to each polymer network. Cyto- compatibility was confirmed by neutral red assay. Human cell invasion was prevented by hydrogel membranes and histological sections revealed that the biomaterial exhibited a barrier effect in contact with soft gingival tissue.
Center for Education and Research on Macromolecules (CERM) ; Complex and Entangled Systems from Atoms to Materials (CESAM)
The Erasumus Mundus project "Nanofar" ; The Euronanomed II project "POsTURE"
Researchers
http://hdl.handle.net/2268/228644
10.1016/j.dental.2018.09.017

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