Article (Scientific journals)
Infrared spectroscopy as a novel tool to diagnose onychomycosis
De Bruyne, Sander; Speeckaert, Reinhart; Boelens, Jerina et al.
2019In British Journal of Dermatology
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Keywords :
Fourier transform infrared spectroscopy; medical laboratory science; mid-infrared absorbance spectroscopy; onychomycosis; vibrational spectroscopy
Abstract :
[en] Background: The determination of causative organisms of onychomycosis is still not optimal. There remains a need for a cheap, fast and easy-to-perform diagnostic tool with highly distinctive capacities. Objectives: To determine whether ATR-FTIR spectroscopy can detect and differentiate causative agents in a culture based, ex vivo nail and in vivo nail model. Methods: A methodological study was conducted. Both the ex vivo nail model and in vivo pilot study were carried out in an academic university hospital. Results: Analysis of cultured fungi revealed spectral differences for dermatophytes (1692- 1606 and 1044-1004 cm-1) and non-dermatophytes/yeasts (973-937 cm-1), confirmed by dendrograms showing an excellent separation between samples from different genera or species. Exploration of dermatophytes, non-dermatophytes and yeasts growing on ex vivo nails exposed prominent differences from 1200 to 900 cm-1. Prediction models resulted in a Accepted Article This article is protected by copyright. All rights reserved. 96.9% accurate classification of uninfected nails and nails infected with dermatophytes, nondermatophytes and yeasts. Overall correct classification rates of 91.0%, 97.7% and 98.6% were obtained for discrimination between dermatophyte, non-dermatophyte and yeast genera or species, respectively. Spectra of in vivo infected and uninfected nails also revealed distinct spectral differences (3000-2811 cm-1, 1043-950 cm-1 and 1676-1553 cm-1), illustrated by two main clusters (uninfected versus infected) on a dendrogram. Conclusions: Our data suggest that ATR-FTIR spectroscopy may be a promising, fast and accurate method to determine onychomycosis, including identification of the causative organism, bypassing the need for lengthy fungal cultures.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
De Bruyne, Sander;  Ghent university hospital > Laboratory medecine
Speeckaert, Reinhart
Boelens, Jerina;  Ghent University Hospital > Clinical microbiology
Hayette, Marie-Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Bactériologie, mycologie, parasitologie, virologie
Speeckaert, Marijn;  Ghent University Hospital > Nephrology
Delanghe, Joris
Language :
Title :
Infrared spectroscopy as a novel tool to diagnose onychomycosis
Alternative titles :
[fr] La spectroscopie infra-rouge: un nouvel outil diagnostique pour les onychomycoses
Publication date :
Journal title :
British Journal of Dermatology
Publisher :
Wiley, Oxford, United Kingdom
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 15 October 2018


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