Reference : Interaction between the barley allelochemical compounds gramine and hordenine and art...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/228166
Interaction between the barley allelochemical compounds gramine and hordenine and artificial lipid bilayers mimicking the plant plasma membrane
English
Lebecque, Simon mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes >]
Crowet, Jean-Marc [> >]
Lins, Laurence mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes >]
Delory, Benjamin [> >]
du Jardin, Patrick mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Ingénierie des productions végétales et valorisation >]
Fauconnier, Marie-Laure mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes >]
Deleu, Magali mailto [Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Chimie des agro-biosystèmes >]
2018
Scientific Reports
Nature Publishing Group
8
Art. n°8
Yes (verified by ORBi)
International
2045-2322
United Kingdom
[en] Some plants affect the development of neighbouring plants by releasing secondary metabolites
into their environment. This phenomenon is known as allelopathy and is a potential tool for weed management within the framework of sustainable agriculture. While many studies have investigated the mode of action of various allelochemicals (molecules emitted by allelopathic plants), little attention has been paid to their initial contact with the plant plasma membrane (PPM). In this paper, this key step is explored for two alkaloids, gramine and hordenine, that are allelochemicals from barley. Using in vitro bioassays, we first showed that gramine has a greater toxicity than hordenine towards a weed commonly found in northern countries (Matricaria recutita L.). Then, isothermal titration calorimetry was used to show that these alkaloids spontaneously interact with lipid bilayers that mimic the PPM.
The greater impact of gramine on the thermotropic behaviour of lipids compared to hordenine was established by means of infrared spectroscopy. Finally, the molecular mechanisms of these interactions were explored with molecular dynamics simulations. The good correlation between phytotoxicity and the ability to disturb lipid bilayers is discussed. In this study, biophysical tools were used for the first time to investigate the interactions of allelochemicals with artificial PPM.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Belgian Program on Interuniversity Attraction Poles initiated by the Federal Office for Scientific, Technical and Cultural Affairs ; Liège Université ; Consortium des Équipements de Calcul Intensif (CÉCI)
PDR grant T.1003.14 / IAP P7/44 iPros) / Action de Recherche Concertée-Project FIELD / Grant No. 2.5020.11
Researchers
http://hdl.handle.net/2268/228166
10.1038/s41598-018-28040-6
https://www.nature.com/articles/s41598-018-28040-6

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