Detection of plant protein in adulterated milk using nontargeted nano- high- performance liquid chromatography– tandem mass spectroscopy combined with principal component analysis
high-speed centrifugation; milk adulteration; plant protein
Résumé :
[en] The objective of this study was to detect plant protein adulterated in fluid milk using nano- high- performance liquid chromatography (HPLC)–tandem mass spectroscopy (LC- MS/MS) combined with proteomics. Unadulterated milk and samples adulterated with soy protein, pea protein, hydrolyzed wheat protein, and hydrolyzed rice protein were prepared, with plant protein level ranged from 0.5% to 8% in total protein. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS- PAGE) gels clearly revealed that centrifugation at 20,000 g for 60 min would reduce band intensity of casein and albumin in milk. Results of nano- HPLC- MS/MS indicated the major proteins of soy (β- conglycinin, glycinin), pea (vincilin, convicilin, legumin), and wheat (glutenin and gliadin) in adulterated milks, allowing detection of soy protein and hydrolyzed wheat protein at the level above 0.5% in total protein and pea protein at the level of 2 and 4%. No rice protein was identified in milk samples adulterated with hydrolyzed rice protein. Combined with principal component analysis, nano- HPLC- MS/MS could discriminate all the adulterated samples from authentic milk. This study demonstrated the feasibility of nano- HPLC- MS/MS on the detection of (hydrolyzed) plant protein adulterated in milk.
Disciplines :
Sciences des denrées alimentaires Productions animales & zootechnie
Auteur, co-auteur :
Yang, Jinhui; Chinese Academy of Agricultural Sciences > State Key Laboratory of Animal Nutrition, Institute of Animal Science
Zheng, Nan; Chinese Academy of Agricultural Sciences, Beijing, China
Soyeurt, Hélène ; Université de Liège - ULiège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Modélisation et développement
Yang; Anhui Academy of Agricultural Sciences, Hefei, China
Wang, Jiaqi; hinese Academy of Agricultural Sciences, Beijing, China
Langue du document :
Anglais
Titre :
Detection of plant protein in adulterated milk using nontargeted nano- high- performance liquid chromatography– tandem mass spectroscopy combined with principal component analysis
Aaslyng, M. D., Martens, M., Poll, L., Nielsen, P. M., Flyge, H., & Larsen, L. M. (1998). Chemical and sensory characterization of hydrolyzed vegetable protein, a savory flavoring. Journal of Agriculture and Food Chemistry, 46, 481–489. https://doi.org/10.1021/jf970556e
Cordewener, J. H. G., Luykx, D. M. A. M., Frankhuizen, R., Bremer, M. G. E. G., Hooijerink, H., & America, A. H. P. (2009). Untargeted LC-Q-TOF mass spectrometry method for the detection of adulterations in skimmed-milk powder. Journal of Separation Science, 32, 1216–1223. https://doi.org/10.1002/jssc.200800568
Du, L., Lu, W., Cai, Z., Bao, L., Hartmann, C., Gao, B., & Yu, L. (2018). Rapid detection of milk adulteration using intact protein flow injection mass spectrometric fingerprints combined with chemometrics. Food Chemistry, 240, 573–578. https://doi.org/10.1016/j.foodchem.2017.07.107
Fox, P. F., Uniacke-Lowe, T., McSweeney, P. L. H., & O'Mahony, J. A. (2015). Dairy chemistry and biochemistry (pp. 145–239). Basel, Switzerland: Springer International. https://doi.org/10.1007/978-3-319-14892-2
Garcia-Molina, M. D., Muccilli, V., Saletti, R., Foti, S., Masci, S., & Barro, F. (2017). Comparative proteomic analysis of two transgenic low-gliadin wheat lines and non-transgenic wheat control. Journal of Proteomics, 165, 102–112. https://doi.org/10.1016/j.jprot.2017.06.010
Haasnoot, W., & du Pre, J. G. (2007). Luminex-based triplex immunoassay for the simultaneous detection of soy, pea, and soluble wheat proteins in milk powder. Journal of Agricultural and Food Chemistry, 55, 3771–3777. https://doi.org/10.1021/jf063281o
IDF (2014). Milk products-Determination of nitrogen content-Part 1: Kjeldahl principle and crude protein calculation. IDF Standard, 020-1
Jablonski, J. E., Moore, J. C., & Harnly, J. M. (2014). Nontargeted detection of adulteration of skim milk powder with foreign proteins using UHPLC-UV. Journal of Agriculture and Food Chemistry, 62, 5198–5206. https://doi.org/10.1021/jf404924x
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685. https://doi.org/10.1038/227680a0
Lopez-Tapia, J., Garcia-Risco, M. R., Manso, M. A., & Lopez-Fandino, R. (1999). Detection of the presence of soya protein in milk powder by sodium dodecyl sulfate capillary electrophoresis. Journal of Chromatography A, 836, 153–160. https://doi.org/10.1016/S0021-9673(98)00918-2
Lu, W., Liu, J., Gao, B., Lv, X., & Yu, L. L. (2017). Technical note: Nontargeted detection of adulterated plant proteins in raw milk by UPLC-quadrupole time-of-flight mass spectrometric proteomics combined with chemometrics. Journal of Dairy Science, 100, 6980–6986. https://doi.org/10.3168/jds.2017-12574
Luykx, D. M. A. M., Cordewener, J. H. G., Ferranti, P., Frankhuizen, R., Bremer, M. G. E. G., Hooijerink, H., & America, A. H. P. (2007). Identification of plant proteins in adulterated skimmed milk powder by high-performance liquid chromatography-mass spectrometry. Journal of Chromatography A, 1164, 189–197. https://doi.org/10.1016/j.chroma.2007.07.017
Manso, M. A., Cattaneo, T. M., Barzaghi, S., Olieman, C., & Lopez-Fandino, R. (2002). Determination of vegetal proteins in milk powder by sodium dodecyl sulfate-capillary gel electrophoresis: interlaboratory study. Journal of AOAC International, 85, 1090–1095
Moore, J. C., Spink, J., & Lipp, M. (2012). Development and application of a database of food ingredient fraud and economically motivated adulteration from 1980 to 2010. Journal of Food Science, 77, R118–R126. https://doi.org/10.1111/j.1750-3841.2012.02657.x
Nakamura, R., & Teshima, R. (2013). Proteomics-based allergen analysis in plants. Journal of Proteomics, 93, 40–49. https://doi.org/10.1016/j.jprot.2013.03.018
Nascimento, C. F., Santos, P. M., Pereira-Filho, E. R., & Rocha, F. R. P. (2017). Recent advances on determination of milk adulterants. Food Chemistry, 221, 1232–1244. https://doi.org/10.1016/j.foodchem.2016.11.034
Scholl, P. F., Farris, S. M., & Mossoba, M. M. (2014). Rapid turbidimetric detection of milk powder adulteration with plant proteins. Journal of Agricultural and Food Chemistry, 62, 1498–1505. https://doi.org/10.1021/jf405617f
Tessier, B., Schweizer, M., Fournier, F., Framboisier, X., Chevalot, I., Vanderesse, R., … Marc, I. (2005). Prediction of the amino acid composition of small peptides contained in a plant protein hydrolysate by LC–MS and CE–MS. Food Research International, 38, 577–584. https://doi.org/10.1016/j.foodres.2004.11.011
Yang, Y. X., Zheng, N., Yang, J. H., Bu, D. P., Wang, J. Q., Ma, L., & Sun, P. (2014). Animal species milk identification by comparison of two-dimensional gel map profile and mass spectrometry approach. International Dairy Journal, 35, 15–20. https://doi.org/10.1016/j.idairyj.2013.09.008
