Reference : Metagenomic assembly of new (sub)polar Cyanobacteria and their associated microbiome ...
Scientific journals : Article
Life sciences : Environmental sciences & ecology
Life sciences : Microbiology
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/227528
Metagenomic assembly of new (sub)polar Cyanobacteria and their associated microbiome from non-axenic cultures.
English
Cornet, Luc mailto [Université de Liège - ULiège > Département des sciences de la vie > Phylogénomique des eucaryotes > >]
Bertrand, Amandine mailto [Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale >]
Hanikenne, Marc mailto [Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale >]
Javaux, Emmanuelle mailto [Université de Liège - ULiège > Département de géologie > Paléobiogéologie - Paléobotanique - Paléopalynologie (PPP) >]
Wilmotte, Annick mailto [Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes >]
Baurain, Denis mailto [Université de Liège - ULiège > Département des sciences de la vie > Phylogénomique des eucaryotes >]
2018
Microbial Genomics
Yes (verified by ORBi)
International
2057-5858
England
[en] Cyanobacteria form one of the most diversified phyla of Bacteria. They are important ecologically as primary producers, for Earth evolution and biotechnological applications. Yet, Cyanobacteria are notably difficult to purify and grow axenically, and most strains in culture collections contain heterotrophic bacteria that were probably associated with Cyanobacteria in the environment. Obtaining cyanobacterial DNA without contaminant sequences is thus a challenging and time-consuming task. Here, we describe a metagenomic pipeline that enables the easy recovery of genomes from non-axenic cultures. We tested this pipeline on 17 cyanobacterial cultures from the BCCM/ULC public collection and generated novel genome sequences for 12 polar or subpolar strains and three temperate ones, including three early-branching organisms that will be useful for phylogenomics. In parallel, we assembled 31 co-cultivated bacteria (12 nearly complete) from the same cultures and showed that they mostly belong to Bacteroidetes and Proteobacteria, some of them being very closely related in spite of geographically distant sampling sites.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Politique Scientifique Fédérale (Belgique) = Belgian Federal Science Policy ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Université de Liège (Fédération Wallonie-Bruxelles) ; CECI ; Tier-1
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/227528
10.1099/mgen.0.000212
FP7 ; 308074 - ELITE - Early Life Traces, Evolution, and Implications for Astrobiology

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