Reference : Membrane topology of the Escherichia coli AmpG permease required for recycling of cel...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Microbiology
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/22696
Membrane topology of the Escherichia coli AmpG permease required for recycling of cell wall anhydromuropeptides and AmpC beta-lactamase induction.
English
Chahboune, Aicha [> > > >]
Decaffmeyer, Marc [> > > >]
Brasseur, Robert mailto [Université de Liège - ULiège > > Gembloux Agro-Bio Tech >]
Joris, Bernard mailto [Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines >]
2005
Antimicrobial Agents and Chemotherapy
American Society for Microbiology (ASM)
49
3
1145-9
Yes (verified by ORBi)
International
0066-4804
1098-6596
Washington
DC
[en] Amino Acid Sequence ; Bacterial Proteins/biosynthesis/chemistry/physiology ; Base Sequence ; Cell Membrane/enzymology ; Cell Wall/metabolism ; Escherichia coli/metabolism ; Membrane Transport Proteins/chemistry/physiology ; Molecular Sequence Data ; Peptidoglycan/metabolism ; beta-Lactamases/biosynthesis
[en] Escherichia coli, and presumably most other gram-negative bacteria, possesses an efficient protein machinery for recycling its peptidoglycan during cell growth. The major recycled peptidoglycan product is N-acetylglucosamine-1,6-anhydro-N-acetylmuramic acid-tetrapeptide. Its uptake from the periplasm into the cytoplasm is carried out via the AmpG protein, an intrinsic membrane protein. In gram-negative bacteria carrying an ampC beta-lactamase-inducible gene on their chromosomes, the induction mechanism is directly linked to peptidoglycan recycling. After identification of the different putative hydrophobic segments by computing, the AmpG topology was experimentally determined by using beta-lactamase fusion. In the proposed model, AmpG contains 10 transmembrane segments and two large cytoplasmic loops.
Researchers ; Professionals
http://hdl.handle.net/2268/22696
also: http://hdl.handle.net/2268/63509
10.1128/AAC.49.3.1145-1149.2005

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