Article (Scientific journals)
Functional characteristics of TauA binding protein from TauABC Escherichia coli system
Javaux, Cédric; Joris, Bernard; De Witte, Philippe
2007In Protein Journal, 26 (4), p. 231-238
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Keywords :
dialysis studies; E. coli ABC transporter; periplasmic binding protein; TauA; taurine
Abstract :
[en] Although TauA shares few common characteristics with other known periplasmic binding protein, TauA is a putative periplasmic binding protein, part of tauABCD gene cluster involved in sulfonate transport in sulphate starvation condition. This protein was expressed in E. coli BL 21 and purified before to assess its binding functionalities. Measurement of K (d) value (mean 11.3 nM) by binding/dialysis studies revealed high affinity and specificity with taurine and also indicated that TauA possessed a unique binding site for its ligand. Comparisons with other periplasmic binding proteins suggests TauA plays a major role in ABC transport system and could be ideal candidate to serve as taurine catcher in biological fluids.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Javaux, Cédric;  UCL
Joris, Bernard ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
De Witte, Philippe;  UCL
Language :
English
Title :
Functional characteristics of TauA binding protein from TauABC Escherichia coli system
Publication date :
June 2007
Journal title :
Protein Journal
ISSN :
1572-3887
Publisher :
Springer, New York, United States - New York
Volume :
26
Issue :
4
Pages :
231-238
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 September 2009

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